PMID- 20477900
OWN - NLM
STAT- MEDLINE
DCOM- 20110418
LR  - 20181201
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 109
IP  - 4
DP  - 2010 Oct
TI  - Lactoferrin-derived peptides and Lactoferricin chimera inhibit virulence factor 
      production and biofilm formation in Pseudomonas aeruginosa.
PG  - 1311-8
LID - 10.1111/j.1365-2672.2010.04751.x [doi]
AB  - AIMS: To investigate the bactericidal activity of lactoferrin-derived peptides 
      and a new LF-derived peptides chimera (LFchimera) against P. aeruginosa and the 
      influence on virulence factors of P. aeruginosa. METHODS AND RESULTS: 
      Lactoferricin (LFcin) and lactoferrampin (LFampin) are highly bioactive peptides 
      isolated from the N-terminal region of lactoferrin (LF) by pepsin digestion. In 
      this study, we designed LFchimera containing LFcin amino acids 17-30 and LFampin 
      amino acids 268-284. Pseudomonas aeruginosa cells were incubated in medium with 
      peptides at different concentrations, and then the assays of viability, 
      pyocyanin, elastase activity and biofilm formation of P. aeruginosa were 
      performed. We found that the concentration-dependent antibactericidal activity 
      and down-regulating pyocyanin, elastase and biofilm formation of LFchimera were 
      significantly stronger than those of LF, LFcin, LFampin or LFcin plus LFampin. 
      CONCLUSIONS: Our results indicated that LF, LFcin, LFampin and LFchimera were 
      potential candidates to combat P. aeruginosa, and LFchimera was the most 
      effective in them. SIGNIFICANCE AND IMPACT OF THE STUDY: The new LFchimera has 
      better activity against P. aeruginosa than LF, LFcin and LFampin and may be a 
      promising new compound for treatment of P. aeruginosa infection.
CI  - (c) 2010 The Authors. Journal compilation (c) 2010 The Society for Applied 
      Microbiology.
FAU - Xu, G
AU  - Xu G
AD  - Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College of 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Xiong, W
AU  - Xiong W
FAU - Hu, Q
AU  - Hu Q
FAU - Zuo, P
AU  - Zuo P
FAU - Shao, B
AU  - Shao B
FAU - Lan, F
AU  - Lan F
FAU - Lu, X
AU  - Lu X
FAU - Xu, Y
AU  - Xu Y
FAU - Xiong, S
AU  - Xiong S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100819
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (LFchimera peptide)
RN  - 0 (Peptide Fragments)
RN  - 0 (Peptides)
RN  - 0 (Virulence Factors)
RN  - 0 (lactoferrampin (268-284))
RN  - 0 (lactoferricin (17-30))
RN  - 9OQM399341 (Pyocyanine)
RN  - EC 3.4.21.- (Lactoferrin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/chemistry/*pharmacology
MH  - Antimicrobial Cationic Peptides/chemistry/*pharmacology
MH  - Biofilms/*drug effects
MH  - Lactoferrin/chemistry/pharmacology
MH  - Peptide Fragments/chemistry/pharmacology
MH  - Peptides/chemistry/*pharmacology
MH  - Pseudomonas aeruginosa/*drug effects/pathogenicity/physiology
MH  - Pyocyanine/biosynthesis
MH  - Virulence Factors/*biosynthesis
EDAT- 2010/05/19 06:00
MHDA- 2011/04/19 06:00
CRDT- 2010/05/19 06:00
PHST- 2010/05/19 06:00 [entrez]
PHST- 2010/05/19 06:00 [pubmed]
PHST- 2011/04/19 06:00 [medline]
AID - JAM4751 [pii]
AID - 10.1111/j.1365-2672.2010.04751.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2010 Oct;109(4):1311-8. doi: 10.1111/j.1365-2672.2010.04751.x. 
      Epub 2010 Aug 19.