PMID- 20479065
OWN - NLM
STAT- MEDLINE
DCOM- 20101222
LR  - 20220331
IS  - 1460-2407 (Electronic)
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 16
IP  - 8
DP  - 2010 Aug
TI  - SNP microarray-based 24 chromosome aneuploidy screening demonstrates that 
      cleavage-stage FISH poorly predicts aneuploidy in embryos that develop to 
      morphologically normal blastocysts.
PG  - 590-600
LID - 10.1093/molehr/gaq037 [doi]
AB  - Although selection of chromosomally normal embryos has the potential to improve 
      outcomes for patients undergoing IVF, the clinical impact of aneuploidy screening 
      by fluorescence in situ hybridization (FISH) has been controversial. There are 
      many putative explanations including sampling error due to mosaicism, negative 
      impact of biopsy, a lack of comprehensive chromosome screening, the possibility 
      of embryo self-correction and poor predictive value of the technology itself. 
      Direct analysis of the negative predictive value of FISH-based aneuploidy 
      screening for an embryo's reproductive potential has not been performed. Although 
      previous studies have found that cleavage-stage FISH is poorly predictive of 
      aneuploidy in morphologically normal blastocysts, putative explanations have not 
      been investigated. The present study used a single nucleotide polymorphism (SNP) 
      microarray-based 24 chromosome aneuploidy screening technology to re-evaluate 
      morphologically normal blastocysts that were diagnosed as aneuploid by FISH at 
      the cleavage stage. Mosaicism and preferential segregation of aneuploidy to the 
      trophectoderm (TE) were evaluated by characterization of multiple sections of the 
      blastocyst. SNP microarray technology also provided the first opportunity to 
      evaluate self-correction mechanisms involving extrusion or duplication of 
      aneuploid chromosomes resulting in uniparental disomy (UPD). Of all blastocysts 
      evaluated (n = 50), 58% were euploid in all sections despite an aneuploid FISH 
      result. Aneuploid blastocysts displayed no evidence of preferential segregation 
      of abnormalities to the TE. In addition, extrusion or duplication of aneuploid 
      chromosomes resulting in UPD did not occur. These findings support the conclusion 
      that cleavage-stage FISH technology is poorly predictive of aneuploidy in 
      morphologically normal blastocysts.
FAU - Northrop, L E
AU  - Northrop LE
AD  - Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA.
FAU - Treff, N R
AU  - Treff NR
FAU - Levy, B
AU  - Levy B
FAU - Scott, R T Jr
AU  - Scott RT Jr
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20100517
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
SB  - IM
MH  - *Aneuploidy
MH  - Blastocyst/*cytology/metabolism
MH  - Cell Line
MH  - Cleavage Stage, Ovum/*metabolism
MH  - Embryonic Development
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Maternal Age
MH  - Microarray Analysis/methods
MH  - *Polymorphism, Single Nucleotide
MH  - Pregnancy
MH  - Preimplantation Diagnosis/*methods
PMC - PMC2907218
EDAT- 2010/05/19 06:00
MHDA- 2010/12/24 06:00
PMCR- 2010/05/17
CRDT- 2010/05/19 06:00
PHST- 2010/05/19 06:00 [entrez]
PHST- 2010/05/19 06:00 [pubmed]
PHST- 2010/12/24 06:00 [medline]
PHST- 2010/05/17 00:00 [pmc-release]
AID - gaq037 [pii]
AID - 10.1093/molehr/gaq037 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2010 Aug;16(8):590-600. doi: 10.1093/molehr/gaq037. Epub 2010 May 
      17.