PMID- 20479374
OWN - NLM
STAT- MEDLINE
DCOM- 20100610
LR  - 20151119
IS  - 1538-361X (Electronic)
IS  - 0886-4470 (Linking)
VI  - 136
IP  - 5
DP  - 2010 May
TI  - Biofilm detection with hematoxylin-eosin staining.
PG  - 453-6
LID - 10.1001/archoto.2010.62 [doi]
AB  - OBJECTIVE: To demonstrate that hematoxylin-eosin staining can be used to detect 
      the presence of bacterial biofilm in patients with chronic rhinosinusitis (CRS). 
      DESIGN: A prospective study. SETTING: The University of Southern California 
      University Hospital and the Department of Otolaryngology-Head and Neck Surgery, 
      University of Southern California, Keck School of Medicine, Los Angeles. 
      PATIENTS: A total of 34 patients: 24 undergoing endoscopic sinus surgery for CRS 
      and 10 undergoing septoplasty with or without turbinate reduction with no history 
      of sinusitis, were enrolled in the study. MAIN OUTCOME MEASURES: Contiguous 
      sections from patient samples were examined by both hematoxylin-eosin staining 
      and fluorescence in situ hybridization (FISH) with the bacterial-specific probe 
      EUB338 for evidence of bacterial biofilm. RESULTS: Biofilm was detected by 
      hematoxylin-eosin staining in 15 of 24 patients with CRS and 1 of 10 control 
      patients. In all cases, hematoxylin-eosin staining was found to be an accurate 
      predictor of the presence or absence of biofilm using FISH as a control standard. 
      CONCLUSION: Hematoxylin-eosin staining of surgical specimens is a reliable and 
      available method for the detection of bacterial biofilm in chronic infectious 
      disease.
FAU - Hochstim, Christian J
AU  - Hochstim CJ
AD  - Department of Otolaryngology-Head and Neck Surgery, Keck School of Medicine, 
      University of Southern California, Los Angeles, Los Angeles, CA 90033, USA.
FAU - Choi, Judy Yujin
AU  - Choi JY
FAU - Lowe, Derek
AU  - Lowe D
FAU - Masood, Rizwan
AU  - Masood R
FAU - Rice, Dale H
AU  - Rice DH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Otolaryngol Head Neck Surg
JT  - Archives of otolaryngology--head & neck surgery
JID - 8603209
RN  - TDQ283MPCW (Eosine Yellowish-(YS))
RN  - YKM8PY2Z55 (Hematoxylin)
SB  - IM
MH  - *Biofilms
MH  - Case-Control Studies
MH  - Endoscopy
MH  - Eosine Yellowish-(YS)
MH  - Hematoxylin
MH  - Humans
MH  - Prospective Studies
MH  - Rhinitis/*microbiology/surgery
MH  - Sinusitis/*microbiology/surgery
MH  - Staining and Labeling/*methods
EDAT- 2010/05/19 06:00
MHDA- 2010/06/11 06:00
CRDT- 2010/05/19 06:00
PHST- 2010/05/19 06:00 [entrez]
PHST- 2010/05/19 06:00 [pubmed]
PHST- 2010/06/11 06:00 [medline]
AID - 136/5/453 [pii]
AID - 10.1001/archoto.2010.62 [doi]
PST - ppublish
SO  - Arch Otolaryngol Head Neck Surg. 2010 May;136(5):453-6. doi: 
      10.1001/archoto.2010.62.