PMID- 20479529
OWN - NLM
STAT- MEDLINE
DCOM- 20101129
LR  - 20131121
IS  - 1878-3627 (Electronic)
IS  - 0922-6028 (Linking)
VI  - 28
IP  - 3
DP  - 2010
TI  - In vitro differentiation of GABAergic cells from bone marrow stromal cells using 
      potassium chloride as inducer.
PG  - 367-77
LID - 10.3233/RNN-2010-0539 [doi]
AB  - Bone marrow stromal cells (BMSCs) are multipotent and can be induced to 
      differentiate into different lineages including osteogenic, chondrogenic, 
      myogenic and neuronal phenotypes. BMSCs were reported to be a suitable option for 
      regenerative medical research. In this study, BMSCs were induced into GABAergic 
      phenotypes using beta-mercaptoethanol (betaME) and retinoic acid (RA) as 
      preinducers followed by potassium chloride as inducer, and were evaluated by 
      fibronectin and Oct-4. The percentages of cells immunoreactive for nestin, 
      neurofilaments (NF 68, NF 160, and NF 200), GABA and GABA synthesizing and 
      packaging enzymes (GAD1, GAD2, VGAT) were used for evaluating GABAergic 
      differentiation. RT-PCR was used for confirming the expression of these enzymes. 
      The differentiated cells were loaded and unloaded with FM1-43 in order to assess 
      the functionality of the cells. At the preinduction stage, the cells 
      downregulated fibronectin and Oct-4, and expressed neuronal markers. At the 
      induction stage, the preinduced cells transdifferentiated into GABAergic cells, 
      as confirmed by immunohistochemistry and RT-PCR. The GABAergic cells were stained 
      and destained with FM1-43. Therefore, the two-stage induction protocol resulted 
      in transdifferentiation of BMSCs into GABAergic cells with synaptic release upon 
      stimulation.
FAU - Gharibani, Payam Mohammad
AU  - Gharibani PM
AD  - Department of Anatomical Sciences, Tarbiat Modares University, School of Medical 
      Sciences, Tehran, Iran.
FAU - Tiraihi, Taki
AU  - Tiraihi T
FAU - Arabkheradmand, Jalil
AU  - Arabkheradmand J
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Restor Neurol Neurosci
JT  - Restorative neurology and neuroscience
JID - 9005499
RN  - 0 (Nerve Tissue Proteins)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - 660YQ98I10 (Potassium Chloride)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/*drug effects/metabolism
MH  - Brain Diseases/metabolism/physiopathology/therapy
MH  - Cell Culture Techniques
MH  - Cell Differentiation/*drug effects/physiology
MH  - Cell Transplantation/methods
MH  - Cells, Cultured
MH  - Interneurons/*cytology/metabolism/transplantation
MH  - Nerve Tissue Proteins/analysis/metabolism
MH  - Potassium Chloride/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stromal Cells/cytology/drug effects/metabolism
MH  - gamma-Aminobutyric Acid/*metabolism
EDAT- 2010/05/19 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/05/19 06:00
PHST- 2010/05/19 06:00 [entrez]
PHST- 2010/05/19 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - Q541813300601042 [pii]
AID - 10.3233/RNN-2010-0539 [doi]
PST - ppublish
SO  - Restor Neurol Neurosci. 2010;28(3):367-77. doi: 10.3233/RNN-2010-0539.