PMID- 20480522
OWN - NLM
STAT- MEDLINE
DCOM- 20100826
LR  - 20211020
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 16
IP  - 19
DP  - 2010 May 21
TI  - Effect of 5-aza-2'-deoxycytidine on immune-associated proteins in exosomes from 
      hepatoma.
PG  - 2371-7
AB  - AIM: To study the effect of 5-aza-2'-deoxycytidine (5-aza-CdR) on heat shock 
      protein 70 (HSP70), human leucocyte antigen-I (HLA-I) and NY-ESO-1 proteins in 
      exosomes produced by hepatoma cells, HepG2 and Hep3B. METHODS: Exosomes derived 
      from HepG(2) and Hep3B cells treated with or without 5-aza-CdR were isolated and 
      purified by ultrafiltration centrifugation and sucrose gradient 
      ultracentrifugation. The number of exosomes was counted under electron 
      microscope. Concentration of proteins in exosomes was measured by bicinchoninic 
      acid protein assay. Expression of HSP70, HLA-I and NY-ESO-1 proteins in exosomes 
      was detected by Western blotting and immunoelectron microscopy. mRNA expression 
      of p53 gene was detected by reverse transcription polymerase chain reaction. 
      RESULTS: The mRNA expression of p53 gene was increased in both hepatoma cell 
      lines after treatment with 5-aza-CdR. The number of exosomes and the 
      concentration of total proteins in exosomes were increased significantly after 
      treatment with 5-aza-CdR (P < 0.05). After treatment with 5-aza-CdR, 
      immunoelectron microscopy and Western blotting showed that the HSP70, HLA-I and 
      NY-ESO-1 proteins were increased in exosomes produced by both hepatoma cell 
      lines. CONCLUSION: 5-Aza-CdR, an inhibitor of DNA methyltransferase, can increase 
      exosomes produced by hepatoma cells and immune-associated protein component of 
      exosomes, which may be mediated by p53 gene up-regulation and 5-aza-CdR 
      demethylation.
FAU - Xiao, Wen-Hua
AU  - Xiao WH
AD  - Department of Oncology, First Affiliated Hospital, Chinese PLA General Hospital, 
      Beijing 100048, China. w_hxiao@hotmail.com
FAU - Sanren, Gao-Wa
AU  - Sanren GW
FAU - Zhu, Jian-Hua
AU  - Zhu JH
FAU - Li, Qiu-Wen
AU  - Li QW
FAU - Kang, Huan-Rong
AU  - Kang HR
FAU - Wang, Ru-Liang
AU  - Wang RL
FAU - Song, Lin-Ping
AU  - Song LP
FAU - Ye, Ming
AU  - Ye M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (CTAG1B protein, human)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (HLA Antigens)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (TP53 protein, human)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 776B62CQ27 (Decitabine)
RN  - EC 2.1.1.- (DNA Modification Methylases)
RN  - M801H13NRU (Azacitidine)
SB  - IM
MH  - Antigens, Neoplasm/metabolism
MH  - Azacitidine/*analogs & derivatives/pharmacology
MH  - Blotting, Western
MH  - Carcinoma, Hepatocellular/enzymology/genetics/*immunology
MH  - Centrifugation, Density Gradient
MH  - DNA Modification Methylases/*antagonists & inhibitors/metabolism
MH  - Decitabine
MH  - Enzyme Inhibitors/*pharmacology
MH  - Exosomes/*drug effects/enzymology/immunology
MH  - HLA Antigens/metabolism
MH  - HSP70 Heat-Shock Proteins/metabolism
MH  - Hep G2 Cells
MH  - Humans
MH  - Liver Neoplasms/enzymology/genetics/*immunology
MH  - Membrane Proteins/metabolism
MH  - Microscopy, Immunoelectron
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Suppressor Protein p53/genetics
MH  - Up-Regulation
PMC - PMC2874141
EDAT- 2010/05/19 06:00
MHDA- 2010/08/27 06:00
PMCR- 2010/05/21
CRDT- 2010/05/19 06:00
PHST- 2010/05/19 06:00 [entrez]
PHST- 2010/05/19 06:00 [pubmed]
PHST- 2010/08/27 06:00 [medline]
PHST- 2010/05/21 00:00 [pmc-release]
AID - 10.3748/wjg.v16.i19.2371 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2010 May 21;16(19):2371-7. doi: 10.3748/wjg.v16.i19.2371.