PMID- 20482897
OWN - NLM
STAT- MEDLINE
DCOM- 20100721
LR  - 20211020
IS  - 1471-2180 (Electronic)
IS  - 1471-2180 (Linking)
VI  - 10
DP  - 2010 May 20
TI  - Phylogenetic analysis and molecular characteristics of seven variant Chinese 
      field isolates of PRRSV.
PG  - 146
LID - 10.1186/1471-2180-10-146 [doi]
AB  - BACKGROUND: Porcine reproductive and respiratory syndrome (PRRS) has now been 
      widely recognized as an economically important disease. The objective of this 
      study was to compare the molecular and biological characteristics of porcine 
      reproductive and respiratory syndrome virus (PRRSV) field isolates in China to 
      those of the modified live virus (MLV) PRRS vaccine and its parent strain (ATCC 
      VR2332). RESULTS: Five genes (GP2, GP3, GP4, GP5 and NSP2) of seven isolates of 
      PRRSV from China, designated LS-4, HM-1, HQ-5, HQ-6, GC-2, GCH-3 and ST-7/2008, 
      were sequenced and analyzed. Phylogenetic analyses based on the nucleotide 
      sequence of the ORF2-5 and NSP2 showed that the seven Chinese isolates belonged 
      to the same genetic subgroup and were related to the North American PRRSV 
      genotype. Comparative analysis with the relevant sequences of another Chinese 
      isolate (BJ-4) and North American (VR2332 and MLV) viruses revealed that these 
      isolates have 80.8-92.9% homology with VR-2332, and 81.3-98.8% identity with MLV 
      and 80.7-92.9% with BJ-4. All Nsp2 nonstructural protein of these seven isolates 
      exhibited variations (a 29 amino acids deletion) in comparison with other North 
      American PRRSV isolates. Therefore, these isolates were novel strain with unique 
      amino acid composition. However, they all share more than 97% identity with other 
      highly pathogenic Chinese PRRSV strains. Additionally, there are extensive amino 
      acid (aa) mutations in the GP5 protein and the Nsp2 protein when compared with 
      the previous isolates. CONCLUSIONS: These results might be useful to study the 
      genetic diversity of PRRSV in China and to track the infection sources as well as 
      for vaccines development.
FAU - Wang, Chengmin
AU  - Wang C
AD  - National Research Center for Wildlife Born Diseases, Institute of Zoology, 
      Chinese Academy of Sciences, Beijing 100101, China.
FAU - Wu, Bin
AU  - Wu B
FAU - Amer, Said
AU  - Amer S
FAU - Luo, Jing
AU  - Luo J
FAU - Zhang, Hongmei
AU  - Zhang H
FAU - Guo, Yunhai
AU  - Guo Y
FAU - Dong, Guoying
AU  - Dong G
FAU - Zhao, Baohua
AU  - Zhao B
FAU - He, Hongxuan
AU  - He H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100520
PL  - England
TA  - BMC Microbiol
JT  - BMC microbiology
JID - 100966981
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - China
MH  - Cluster Analysis
MH  - Genes, Viral
MH  - Genotype
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - *Polymorphism, Genetic
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*classification/genetics/*isolation & purification
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Swine/*virology
PMC - PMC2889949
EDAT- 2010/05/21 06:00
MHDA- 2010/07/22 06:00
PMCR- 2010/05/20
CRDT- 2010/05/21 06:00
PHST- 2010/02/28 00:00 [received]
PHST- 2010/05/20 00:00 [accepted]
PHST- 2010/05/21 06:00 [entrez]
PHST- 2010/05/21 06:00 [pubmed]
PHST- 2010/07/22 06:00 [medline]
PHST- 2010/05/20 00:00 [pmc-release]
AID - 1471-2180-10-146 [pii]
AID - 10.1186/1471-2180-10-146 [doi]
PST - epublish
SO  - BMC Microbiol. 2010 May 20;10:146. doi: 10.1186/1471-2180-10-146.