PMID- 20483646
OWN - NLM
STAT- MEDLINE
DCOM- 20101019
LR  - 20100719
IS  - 1096-7206 (Electronic)
IS  - 1096-7192 (Linking)
VI  - 100
IP  - 4
DP  - 2010 Aug
TI  - Differential expression of phosphofructokinase-1 isoforms correlates with the 
      glycolytic efficiency of breast cancer cells.
PG  - 372-8
LID - 10.1016/j.ymgme.2010.04.006 [doi]
AB  - Cancer cells are characterized by increased aerobic glycolysis, which correlates 
      with a negative prognosis. Although this correlation is well known, the mechanism 
      of the elevated rate of glycolysis in cancer and the role of glycolytic enzymes 
      have yet to be determined. The present work aims to evaluate the activity of the 
      major enzymes that regulate glycolysis in breast cancer cell lines of varying 
      aggressiveness. MCF10A, MCF-7 and MDA-mb-231 are human breast-derived cell lines 
      with non-tumorigenic, tumorigenic and metastatic profiles, respectively. These 
      cell lines have increasing degrees of glycolytic efficiency, i.e., lactate 
      produced per glucose consumed, corresponding to their metastatic potential. 
      Although, there are no differences in phosphofructokinase (PFK) or pyruvate 
      kinase (PK) activities, the activity of hexokinase (HK) activity is higher in 
      both tumorigenic cell lines compared to MCF10A cells. No difference in HK 
      activity is observed between MCF-7 and MDA-mb-231 cells, suggesting that the 
      difference in their glycolytic efficiency could not be attributed to this enzyme. 
      However, we find that expression of the PFK-L isoform directly and strongly 
      correlates with aggressiveness and glycolytic efficiency in these cell lines. 
      Thus, we conclude that glycolytic efficiency, which is important for the survival 
      of cancer cells, depends primarily on the preferential expression of PFK-L over 
      the M and P isoforms.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Zancan, Patricia
AU  - Zancan P
AD  - Laboratorio de Oncobiologia Molecular - LabOMol, Faculdade de Farmacia, UFRJ, 
      Brazil. pzancan@ufrj.br
FAU - Sola-Penna, Mauro
AU  - Sola-Penna M
FAU - Furtado, Cristiane Marques
AU  - Furtado CM
FAU - Da Silva, Daniel
AU  - Da Silva D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100418
PL  - United States
TA  - Mol Genet Metab
JT  - Molecular genetics and metabolism
JID - 9805456
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
SB  - IM
MH  - Breast Neoplasms/*enzymology/*genetics
MH  - Cell Line, Tumor
MH  - Female
MH  - *Gene Expression Profiling
MH  - *Gene Expression Regulation, Enzymologic
MH  - *Gene Expression Regulation, Neoplastic
MH  - Glycolysis/*genetics
MH  - Humans
MH  - Isoenzymes/genetics/metabolism
MH  - Metabolome/genetics
MH  - Phosphofructokinase-1/*genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
EDAT- 2010/05/21 06:00
MHDA- 2010/10/20 06:00
CRDT- 2010/05/21 06:00
PHST- 2010/03/19 00:00 [received]
PHST- 2010/04/13 00:00 [revised]
PHST- 2010/04/13 00:00 [accepted]
PHST- 2010/05/21 06:00 [entrez]
PHST- 2010/05/21 06:00 [pubmed]
PHST- 2010/10/20 06:00 [medline]
AID - S1096-7192(10)00151-4 [pii]
AID - 10.1016/j.ymgme.2010.04.006 [doi]
PST - ppublish
SO  - Mol Genet Metab. 2010 Aug;100(4):372-8. doi: 10.1016/j.ymgme.2010.04.006. Epub 
      2010 Apr 18.