PMID- 20484859
OWN - NLM
STAT- MEDLINE
DCOM- 20100816
LR  - 20210702
IS  - 1881-7122 (Electronic)
IS  - 0007-5124 (Linking)
VI  - 59
IP  - 2
DP  - 2010
TI  - Effects of strain differences and vehicles on results of local lymph node assays.
PG  - 245-9
AB  - The Local Lymph Node Assay (LLNA) is now regarded as the worldwide standard. The 
      analysis of accumulated LLNA data reveals that the animal strains and vehicles 
      employed are likely to affect LLNA results. Here we show that an obvious strain 
      difference in the local lymph node response was observed between DMSO-treated 
      CBA/CaOlaHsd and CBA/CaHsdRcc mice. We also show that a vehicle difference in the 
      response was observed when CBA/CaHsdRcc mice were exposed to 6 vehicles; 4:1 v/v 
      acetone/olive oil (AOO), ethanol/water (70% EtOH), N,N-dimethylformamide (DMF), 
      2-butanone (BN), propylene glycol (PG), and dimethylsulfoxide (DMSO). The dpm/LN 
      level was lowest in the 70% EtOH group and highest in the DMSO group. When 
      alpha-hexylcinnamaldehyde (HCA) was used as a sensitizer for the LLNA, HCA was a 
      weak sensitizer when AOO or DMSO was used as a vehicle, but a moderate sensitizer 
      when the other 4 vehicles were used. This study showed that there are vehicle 
      differences in the local lymph node response (dpm/LN level) in the LLNA and that 
      the sensitization potency of HCA may be classified in different categories when 
      using different vehicles. This suggests that careful consideration should be 
      exercised in selecting a vehicle for the LLNA. A further comprehensive study will 
      be needed to investigate why vehicle differences are observed in the LLNA.
FAU - Anzai, Takayuki
AU  - Anzai T
AD  - Showa University School of Medicine, Tokyo, Japan.
FAU - Ullmann, Ludwig G
AU  - Ullmann LG
FAU - Hayashi, Daisuke
AU  - Hayashi D
FAU - Satoh, Tetsuo
AU  - Satoh T
FAU - Kumazawa, Takeshi
AU  - Kumazawa T
FAU - Sato, Keizo
AU  - Sato K
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Exp Anim
JT  - Experimental animals
JID - 9604830
RN  - 0 (Allergens)
RN  - 0 (Pharmaceutical Vehicles)
RN  - 7864XYD3JJ (Acrolein)
RN  - SR60A3XG0F (cinnamaldehyde)
SB  - IM
MH  - Acrolein/*analogs & derivatives/pharmacology
MH  - Allergens/immunology/*pharmacology
MH  - Animals
MH  - Dermatitis, Allergic Contact/etiology/immunology
MH  - Female
MH  - Immunization
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/*drug effects/immunology/pathology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Pharmaceutical Vehicles/*pharmacology
MH  - Species Specificity
EDAT- 2010/05/21 06:00
MHDA- 2010/08/17 06:00
CRDT- 2010/05/21 06:00
PHST- 2010/05/21 06:00 [entrez]
PHST- 2010/05/21 06:00 [pubmed]
PHST- 2010/08/17 06:00 [medline]
AID - JST.JSTAGE/expanim/59.245 [pii]
AID - 10.1538/expanim.59.245 [doi]
PST - ppublish
SO  - Exp Anim. 2010;59(2):245-9. doi: 10.1538/expanim.59.245.