PMID- 20484864
OWN - NLM
STAT- MEDLINE
DCOM- 20100923
LR  - 20190911
IS  - 1347-8648 (Electronic)
IS  - 1347-8613 (Linking)
VI  - 113
IP  - 2
DP  - 2010
TI  - Sustained exposure to 3,4-methylenedioxymethamphetamine induces the augmentation 
      of exocytotic serotonin release in rat organotypic raphe slice cultures.
PG  - 197-201
AB  - 3,4-Methylenedioxymethamphetamine (MDMA) causes serotonin efflux via serotonin 
      transporter. Recently, we have reported that sustained exposure to MDMA induced 
      an augmentation of serotonin release in rat raphe serotonergic slice cultures. 
      Here we investigated the mechanism of augmented serotonin release from the slice 
      cultures. Sustained MDMA exposure had no effect on MDMA-induced serotonin efflux 
      in the synaptosomal fraction, whereas either tetrodotoxin, calcium channel 
      inhibitors, or AMPA-receptor antagonists significantly attenuated the augmented 
      serotonin release. These results suggest that the increase in Ca(2+)-dependent 
      exocytotic serotonin release is mediated through activation of AMPA receptors and 
      responsible for the sustained MDMA-induced augmentation of serotonin release.
FAU - Nagayasu, Kazuki
AU  - Nagayasu K
AD  - Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, 
      Kyoto University, Kyoto, Japan.
FAU - Kitaichi, Maiko
AU  - Kitaichi M
FAU - Shirakawa, Hisashi
AU  - Shirakawa H
FAU - Nakagawa, Takayuki
AU  - Nakagawa T
FAU - Kaneko, Shuji
AU  - Kaneko S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100519
PL  - Japan
TA  - J Pharmacol Sci
JT  - Journal of pharmacological sciences
JID - 101167001
RN  - 0 (Receptors, AMPA)
RN  - 0 (Serotonin Plasma Membrane Transport Proteins)
RN  - 333DO1RDJY (Serotonin)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/metabolism
MH  - Exocytosis/*drug effects
MH  - N-Methyl-3,4-methylenedioxyamphetamine/administration & dosage/*pharmacology
MH  - Organ Culture Techniques
MH  - Raphe Nuclei/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, AMPA/physiology
MH  - Serotonin/*metabolism
MH  - Serotonin Plasma Membrane Transport Proteins/physiology
MH  - Stimulation, Chemical
EDAT- 2010/05/21 06:00
MHDA- 2010/09/24 06:00
CRDT- 2010/05/21 06:00
PHST- 2010/05/21 06:00 [entrez]
PHST- 2010/05/21 06:00 [pubmed]
PHST- 2010/09/24 06:00 [medline]
AID - JST.JSTAGE/jphs/10075SC [pii]
AID - 10.1254/jphs.10075sc [doi]
PST - ppublish
SO  - J Pharmacol Sci. 2010;113(2):197-201. doi: 10.1254/jphs.10075sc. Epub 2010 May 
      19.