PMID- 20488263
OWN - NLM
STAT- MEDLINE
DCOM- 20101004
LR  - 20100721
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 28
IP  - 32
DP  - 2010 Jul 19
TI  - Development of an 8-plex Luminex assay to detect swine cytokines for vaccine 
      development: assessment of immunity after porcine reproductive and respiratory 
      syndrome virus (PRRSV) vaccination.
PG  - 5356-64
LID - 10.1016/j.vaccine.2010.05.016 [doi]
AB  - A Luminex (Luminex Corp., Austin, TX) multiplex swine cytokine assay was 
      developed to measure 8 cytokines simultaneously in pig serum for use in 
      assessment of vaccine candidates. The fluorescent microsphere immunoassay (FMIA) 
      was tested on archived sera in a porcine reproductive and respiratory syndrome 
      virus (PRRSV) vaccine/challenge study. This FMIA simultaneously detects innate 
      (IL-1 beta, IL-8, IFN-alpha, TNF-alpha, IL-12), regulatory (IL-10), Th1 
      (IFN-gamma) and Th2 (IL-4) cytokines. These proteins were measured to evaluate 
      serum cytokine levels associated with vaccination strategies that provided for 
      different levels of protective immunity against PRRSV. Pigs were vaccinated with 
      a modified-live virus (MLV) vaccine and subsequently challenged with a 
      non-identical PRRSV isolate (93% identity in the glycoprotein (GP5) gene). 
      Protection (as defined by no serum viremia) was observed in the MLV vaccinated 
      pigs after PRRSV challenge but not those vaccinated with killed virus vaccine 
      with adjuvant (KV/ADJ) (99% identity in the GP5 gene to the challenge strain) or 
      non-vaccinates. Significantly elevated levels of IL-12 were observed in the 
      KV/ADJ group compared to MLV vaccinated and control groups. However, this 
      significant increase in serum IL-12 did not correlate with protection against 
      PRRSV viremia. Additional studies using this assay to measure the local cytokine 
      tissue responses may help in defining a protective cytokine response and would be 
      useful for the targeted design of efficacious vaccines, not only for PRRSV, but 
      also for other swine pathogens.
CI  - (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Lawson, Steven
AU  - Lawson S
AD  - Veterinary Science Department, South Dakota State University, North Campus Drive, 
      Brookings, SD 57007, USA.
FAU - Lunney, Joan
AU  - Lunney J
FAU - Zuckermann, Federico
AU  - Zuckermann F
FAU - Osorio, Fernando
AU  - Osorio F
FAU - Nelson, Eric
AU  - Nelson E
FAU - Welbon, Craig
AU  - Welbon C
FAU - Clement, Travis
AU  - Clement T
FAU - Fang, Ying
AU  - Fang Y
FAU - Wong, Susan
AU  - Wong S
FAU - Kulas, Karen
AU  - Kulas K
FAU - Christopher-Hennings, Jane
AU  - Christopher-Hennings J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100518
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Cytokines)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/chemistry
MH  - Cytokines/*analysis/blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fluoroimmunoassay/*methods
MH  - Microspheres
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/prevention & control
MH  - Reproducibility of Results
MH  - Swine
MH  - Viral Envelope Proteins/immunology
MH  - Viral Vaccines/*immunology
EDAT- 2010/05/22 06:00
MHDA- 2010/10/05 06:00
CRDT- 2010/05/22 06:00
PHST- 2010/01/12 00:00 [received]
PHST- 2010/03/23 00:00 [revised]
PHST- 2010/05/04 00:00 [accepted]
PHST- 2010/05/22 06:00 [entrez]
PHST- 2010/05/22 06:00 [pubmed]
PHST- 2010/10/05 06:00 [medline]
AID - S0264-410X(10)00681-X [pii]
AID - 10.1016/j.vaccine.2010.05.016 [doi]
PST - ppublish
SO  - Vaccine. 2010 Jul 19;28(32):5356-64. doi: 10.1016/j.vaccine.2010.05.016. Epub 
      2010 May 18.