PMID- 20488802
OWN - NLM
STAT- MEDLINE
DCOM- 20101203
LR  - 20100812
IS  - 1460-2350 (Electronic)
IS  - 0268-1161 (Linking)
VI  - 25
IP  - 7
DP  - 2010 Jul
TI  - Improving FISH diagnosis for preimplantation genetic aneuploidy screening.
PG  - 1812-7
LID - 10.1093/humrep/deq122 [doi]
AB  - BACKGROUND: In our routine programme of preimplantation genetic aneuploidy 
      screening (PGS) by fluorescence in situ hybridization (FISH), nine chromosomes 
      (13, 15, 16, 17, 18, 21, 22, X and Y) are analysed in two consecutive 
      hybridization rounds. We also perform additional hybridization rounds for these 
      chromosomes, using probes that bind to different loci, for non-conclusive results 
      and for confirmation of certain aneuploidies. The aim of this study was to 
      evaluate the impact of additional hybridization rounds on FISH accuracy. METHODS: 
      This is a retrospective analysis of our FISH data from 1000 PGS cycles performed 
      from December 2007 to December 2008 for various indications. In addition to the 
      hybridization rounds described above, 132 of the embryos diagnosed as 
      chromosomally abnormal were re-analysed on Day 5. RESULTS: A total of 2477 
      embryos were re-hybridized, 1496 due to non-conclusive results and 981 to confirm 
      observed aneuploidies. After re-hybridization, 882 embryos (59%) were then 
      diagnosed as normal, 600 embryos (40.1%) had a clear abnormality and only 14 
      embryos (0.9%) remained non-informative. From the 981 embryos in the latter 
      group, 890 embryos had monosomies and, after re-hybridization 174 embryos (19.6%) 
      were normal and 716 (80.5%) had confirmed monosomies. In contrast, 
      re-hybridization confirmed 90 (98.9%) of the 91 observed trisomies. In addition, 
      Day-5 re-analysis of abnormal embryos showed a higher rate of concordant 
      diagnosis between Day 3 and Day 5 when re-hybridizations had been included on 
      Day-3 (95 versus 82.7%; P= 0.0443), especially for the confirmation of monosomies 
      (82.8 versus 61.0%; P = 0.0087). CONCLUSIONS: Our data indicate that additional 
      hybridization rounds improve the accuracy of the diagnosis, increasing the number 
      of chromosomally normal embryos available for transfer. Re-hybridization with 
      additional probes as a standard approach to PGS could enhance the potential 
      benefits of the technique.
FAU - Mir, P
AU  - Mir P
AD  - PGD Unit, Institut Universitari IVI-Valencia, Plaza Policia Local, 3, 46015 
      Valencia, Spain. pmir@ivi.es
FAU - Rodrigo, L
AU  - Rodrigo L
FAU - Mateu, E
AU  - Mateu E
FAU - Peinado, V
AU  - Peinado V
FAU - Milan, M
AU  - Milan M
FAU - Mercader, A
AU  - Mercader A
FAU - Buendia, P
AU  - Buendia P
FAU - Delgado, A
AU  - Delgado A
FAU - Pellicer, A
AU  - Pellicer A
FAU - Remohi, J
AU  - Remohi J
FAU - Rubio, C
AU  - Rubio C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100519
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
SB  - IM
MH  - *Aneuploidy
MH  - Blastocyst/*ultrastructure
MH  - Chromosome Disorders/*diagnosis
MH  - Chromosomes, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Preimplantation Diagnosis/*methods
MH  - Retrospective Studies
MH  - Sensitivity and Specificity
EDAT- 2010/05/22 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/05/22 06:00
PHST- 2010/05/22 06:00 [entrez]
PHST- 2010/05/22 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - deq122 [pii]
AID - 10.1093/humrep/deq122 [doi]
PST - ppublish
SO  - Hum Reprod. 2010 Jul;25(7):1812-7. doi: 10.1093/humrep/deq122. Epub 2010 May 19.