PMID- 20488853
OWN - NLM
STAT- MEDLINE
DCOM- 20110829
LR  - 20131121
IS  - 1477-0903 (Electronic)
IS  - 0960-3271 (Linking)
VI  - 30
IP  - 5
DP  - 2011 May
TI  - In vitro evaluation of the genotoxicity of ritodrine and verapamil in human 
      lymphocytes.
PG  - 398-405
LID - 10.1177/0960327110372404 [doi]
AB  - The aim of this study was to investigate the genotoxic effects of ritodrine and 
      verapamil on human peripheral lymphocytes in vitro using micronucleus (MN) test. 
      Also, fluorescence in situ hybridization (FISH) with a centromeric probe was 
      performed to determine the origin of the induced MN. Cells were treated with 8.4 
      x 10(-6) M - 25.2 x 10(-4) M concentrations for ritodrine and 0.56 - 11 x 10(-5) 
      M concentrations for verapamil, separately and combined. The MN frequencies 
      showed increase after all treatments, but the difference between treated cells 
      and untreated controls were found to be statistically significant only in the 
      concentration range from 8.4 x 10(-5) M - 4.5 x 10(-4) M for ritodrine, 1.1 - 3.3 
      x 10(-5) M for verapamil, and in combined treatment with concentrations 8.4 x 
      10(-5) M + 1.1 x 10(-5) M for ritodrine and verapamil. The highest tested 
      concentrations of both medicaments showed cytotoxic effect. Both medicaments 
      decreased the nuclear division index (NDI) in tested concentrations. The results 
      of FISH analysis suggest that verapamil, separately or combined with ritodrine, 
      shows to a larger extent aneugenic than clastogenic effect.
FAU - Milosevic-Djordjevic, O
AU  - Milosevic-Djordjevic O
AD  - Faculty of Science, Department of Genetics, University of Kragujevac, Kragujevac, 
      Serbia, Medical Faculty, University of Kragujevac, Kragujevac, Serbia. 
      olivera@kg.ac.rs
FAU - Grujicic, D
AU  - Grujicic D
FAU - Joksic, G
AU  - Joksic G
FAU - Marinkovic, D
AU  - Marinkovic D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100520
PL  - England
TA  - Hum Exp Toxicol
JT  - Human & experimental toxicology
JID - 9004560
RN  - 0 (Mutagens)
RN  - CJ0O37KU29 (Verapamil)
RN  - I0Q6O6740J (Ritodrine)
SB  - IM
MH  - Adult
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Drug Synergism
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*drug effects/ultrastructure
MH  - Micronuclei, Chromosome-Defective/*chemically induced
MH  - Micronucleus Tests
MH  - Molecular Structure
MH  - Mutagens/chemistry/*toxicity
MH  - Ritodrine/chemistry/*toxicity
MH  - Verapamil/chemistry/*toxicity
EDAT- 2010/05/22 06:00
MHDA- 2011/08/30 06:00
CRDT- 2010/05/22 06:00
PHST- 2010/05/22 06:00 [entrez]
PHST- 2010/05/22 06:00 [pubmed]
PHST- 2011/08/30 06:00 [medline]
AID - 0960327110372404 [pii]
AID - 10.1177/0960327110372404 [doi]
PST - ppublish
SO  - Hum Exp Toxicol. 2011 May;30(5):398-405. doi: 10.1177/0960327110372404. Epub 2010 
      May 20.