PMID- 20489032
OWN - NLM
STAT- MEDLINE
DCOM- 20150409
LR  - 20151119
IS  - 1522-1601 (Electronic)
IS  - 0161-7567 (Linking)
VI  - 109
IP  - 2
DP  - 2010 Aug
TI  - Effect of glycogen availability on human skeletal muscle protein turnover during 
      exercise and recovery.
PG  - 431-8
LID - 10.1152/japplphysiol.00108.2009 [doi]
AB  - We examined the effect of carbohydrate (CHO) availability on whole body and 
      skeletal muscle protein utilization at rest, during exercise, and during recovery 
      in humans. Six men cycled at approximately 75% peak O(2) uptake (Vo(2peak)) to 
      exhaustion to reduce body CHO stores and then consumed either a high-CHO (H-CHO; 
      71 + or - 3% CHO) or low-CHO (L-CHO; 11 + or - 1% CHO) diet for 2 days before the 
      trial in random order. After each dietary intervention, subjects received a 
      primed constant infusion of [1-(13)C]leucine and l-[ring-(2)H(5)]phenylalanine 
      for measurements of the whole body net protein balance and skeletal muscle 
      protein turnover. Muscle, breath, and arterial and venous blood samples were 
      obtained at rest, during 2 h of two-legged kicking exercise at approximately 45% 
      of kicking Vo(2peak), and during 1 h of recovery. Biopsy samples confirmed that 
      the muscle glycogen concentration was lower in the L-CHO group versus the H-CHO 
      group at rest, after exercise, and after recovery. The net leg protein balance 
      was decreased in the L-CHO group compared with at rest and compared with the 
      H-CHO condition, which was primarily due to an increase in protein degradation 
      (area under the curve of the phenylalanine rate of appearance: 1,331 + or - 162 
      micromol in the L-CHO group vs. 786 + or - 51 micromol in the H-CHO group, P < 
      0.05) but also due to a decrease in protein synthesis late in exercise. There 
      were no changes during exercise in the rate of appearance compared with rest in 
      the H-CHO group. Whole body leucine oxidation increased above rest in the L-CHO 
      group only and was higher than in the H-CHO group. The whole body net protein 
      balance was reduced in the L-CHO group, largely due to a decrease in whole body 
      protein synthesis. These data extend previous findings by others and demonstrate, 
      using contemporary stable isotope methodology, that CHO availability influences 
      the rates of skeletal muscle and whole body protein synthesis, degradation, and 
      net balance during prolonged exercise in humans.
FAU - Howarth, Krista R
AU  - Howarth KR
AD  - Exercise Metabolism Research Group, Department of Kinesiology, McMaster 
      University, Hamilton, Ontario, Canada.
FAU - Phillips, Stuart M
AU  - Phillips SM
FAU - MacDonald, Maureen J
AU  - MacDonald MJ
FAU - Richards, Douglas
AU  - Richards D
FAU - Moreau, Natalie A
AU  - Moreau NA
FAU - Gibala, Martin J
AU  - Gibala MJ
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20100520
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Biomarkers)
RN  - 0 (Blood Glucose)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Insulin)
RN  - 0 (Muscle Proteins)
RN  - 47E5O17Y3R (Phenylalanine)
RN  - 9005-79-2 (Glycogen)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Adult
MH  - Biomarkers/blood
MH  - Biopsy
MH  - Blood Glucose/metabolism
MH  - Breath Tests
MH  - Chromatography, High Pressure Liquid
MH  - Diet, Carbohydrate-Restricted
MH  - Dietary Carbohydrates/administration & dosage/*metabolism
MH  - *Exercise
MH  - Gas Chromatography-Mass Spectrometry
MH  - Glycogen/*metabolism
MH  - Heart Rate
MH  - Humans
MH  - Insulin/blood
MH  - Leucine/administration & dosage/blood
MH  - Male
MH  - *Muscle Contraction
MH  - Muscle Proteins/*metabolism
MH  - Muscle, Skeletal/blood supply/*metabolism
MH  - Oxidation-Reduction
MH  - Oxygen Consumption
MH  - Phenylalanine/blood
MH  - Pulmonary Ventilation
MH  - Recovery of Function
MH  - Regional Blood Flow
MH  - Time Factors
MH  - Young Adult
EDAT- 2010/05/22 06:00
MHDA- 2015/04/10 06:00
CRDT- 2010/05/22 06:00
PHST- 2010/05/22 06:00 [entrez]
PHST- 2010/05/22 06:00 [pubmed]
PHST- 2015/04/10 06:00 [medline]
AID - japplphysiol.00108.2009 [pii]
AID - 10.1152/japplphysiol.00108.2009 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 2010 Aug;109(2):431-8. doi: 
      10.1152/japplphysiol.00108.2009. Epub 2010 May 20.