PMID- 20490325
OWN - NLM
STAT- MEDLINE
DCOM- 20100820
LR  - 20211020
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 3
IP  - 4
DP  - 2010 Mar 20
TI  - Piwil2 is expressed in various stages of breast cancers and has the potential to 
      be used as a novel biomarker.
PG  - 328-37
AB  - Piwil2, a member of AGO/PIWI family of proteins, has been reported to be 
      expressed in precancerous stem cells (pCSCs), tumor cell lines and various types 
      of human cancers. However, the significance of piwil2 expression in breast cancer 
      has not been investigated. In this study, archival formalin-fixed, 
      paraffin-embedded breast cancer specimens at various developmental stages were 
      prepared as tissue microarrays (TMAs) and examined for the expressions of piwil2, 
      estrogen receptor (ER), progesterone receptor (PR) and a cell proliferation 
      marker Ki67 by immunohistochemical (IHC) staining and human epidermal growth 
      factor receptor 2 (HER2) by fluorescence in situ hybridization (FISH). The 
      correlation of piwil2 expression with ER, PR and Ki67 were analyzed 
      statistically. The piwil2 was detected in all of breast cancer TMA cores. In 
      contrast, ER, PR, HER2, and Ki67 were detected only in 66.1%, 54.5%, 36.0%, and 
      47% of the TMA cores, respectively. Piwil2 was expressed in cytoplasm (Cyt), 
      nucleus (N) or both cytoplasm and nucleus (C-N). The N pattern was less observed 
      in breast precancers, whereas all three patterns were observed in invasive and 
      metastatic cancers. While the Cyt pattern was significantly correlated with ER 
      expression (p = 0.002); N pattern was significantly correlated with Ki67 
      expression (p =0.001). ER and Ki67 expressions were reduced and increased, 
      respectively, with the expression patterns being shifted from Cyt --> C-N --> N. 
      In conclusion, piwil2 is expressed in various stages of breast cancers and has 
      the potential to be used a novel biomarker.
FAU - Liu, James J
AU  - Liu JJ
AD  - Department of Pathology, Ohio State University Medical Center, Columbus, OH 
      43210, USA.
FAU - Shen, Rulong
AU  - Shen R
FAU - Chen, Li
AU  - Chen L
FAU - Ye, Yin
AU  - Ye Y
FAU - He, Gang
AU  - He G
FAU - Hua, Keding
AU  - Hua K
FAU - Jarjoura, David
AU  - Jarjoura D
FAU - Nakano, Toru
AU  - Nakano T
FAU - Ramesh, Ganju K
AU  - Ramesh GK
FAU - Shapiro, Charles L
AU  - Shapiro CL
FAU - Barsky, Sanford H
AU  - Barsky SH
FAU - Gao, Jian-Xin
AU  - Gao JX
LA  - eng
GR  - R01 CA109527/CA/NCI NIH HHS/United States
GR  - UL1 RR025755/RR/NCRR NIH HHS/United States
GR  - CA109527/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100320
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
RN  - 0 (Argonaute Proteins)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (PIWIL2 protein, human)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Progesterone)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Argonaute Proteins
MH  - Biomarkers, Tumor/*analysis
MH  - Breast Neoplasms/genetics/*metabolism
MH  - Female
MH  - Gene Expression
MH  - Gene Expression Profiling
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Ki-67 Antigen/biosynthesis/genetics
MH  - Neoplasm Staging
MH  - Proteins/*metabolism
MH  - Receptor, ErbB-2/biosynthesis/genetics
MH  - Receptors, Estrogen/biosynthesis/genetics
MH  - Receptors, Progesterone/biosynthesis/genetics
MH  - Tissue Array Analysis
PMC - PMC2872741
OTO - NOTNLM
OT  - HER2
OT  - Piwil2
OT  - and Ki67
OT  - breast cancer
OT  - estrogen receptor
OT  - field cancerization
OT  - precancer
OT  - progesterone receptor
EDAT- 2010/05/22 06:00
MHDA- 2010/08/21 06:00
PMCR- 2010/03/20
CRDT- 2010/05/22 06:00
PHST- 2010/02/25 00:00 [received]
PHST- 2010/03/15 00:00 [accepted]
PHST- 2010/05/22 06:00 [entrez]
PHST- 2010/05/22 06:00 [pubmed]
PHST- 2010/08/21 06:00 [medline]
PHST- 2010/03/20 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2010 Mar 20;3(4):328-37.