PMID- 20491433
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20100922
LR  - 20100614
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 82
IP  - 12
DP  - 2010 Jun 15
TI  - Double covalent coupling method for the fabrication of highly sensitive and 
      reusable electrogenerated chemiluminescence sensors.
PG  - 5046-52
LID - 10.1021/ac9029289 [doi]
AB  - A double covalent coupling method for the fabrication of a highly sensitive and 
      reusable electrogenerated chemiluminescence (ECL) chemical sensor for the 
      detection of tertiary amines and ECL aptamer-based (ECL-AB) biosensor for the 
      detection of cocaine is reported. The ECL sensors were constructed by covalent 
      coupling of amino-containing Ru(bpy)(3)(2+) derivatives (Ru1, Ru(bpy)(3)(2+) = 
      tris(2,2'-bipyridyl)ruthenium(II)) or cocaine aptamer-Ru1 to the surface of a 
      paraffin-impregnated graphite electrode that had been covalently modified with a 
      monolayer of 4-aminobenzene sulfonic acid via electrochemical oxidations. ECL 
      performance of the newly developed chemical sensors was evaluated using 
      tri-n-propylamine (TPrA) and metoclopramide (MCP) as model analytes. The sensors 
      exhibited excellent sensitivity, stability, and reproducibility with a detection 
      limit of 30 nM for TPrA and 2.0 nM for MCP, and relative standard deviations 
      (RSDs) of 2.1% over 90 cyclic potential cycles (0 to 1.50 V vs Ag/AgCl) and 2.6% 
      over 45 cycles (0.60 to +1.30 V vs Ag/AgCl) at 400 mV/s for 50 nM TPrA and 200 nM 
      MCP, respectively. For the ECL-AB biosensor, it showed an extremely low detection 
      limit of 10 pM for cocaine, and offered a good selectivity toward cocaine, 
      heroin, and caffeine. This detection limit was about 4-6 orders of magnitude 
      lower than that reported on the basis of alternating current (AC) voltammetry and 
      optical aptamer-based cocaine biosensors. Additionally, the ECL-AB biosensor was 
      highly reusable (RSD = 2.8%, n = 7) and possessed long-term storage stability 
      (96.8% initial ECL recovery over 21 days storage). A binding constant of 4.6 +/- 
      0.3 x 10(9) M(-1) between cocaine and its aptamer was estimated using an ECL 
      based Langmuir isotherm approach. Wide ranging applications of the presently 
      reported strategy in fabricating various chemical sensors or biosensors are 
      expected.
FAU - Sun, Bo
AU  - Sun B
AD  - Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, 
      School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 
      710062, PR China.
FAU - Qi, Honglan
AU  - Qi H
FAU - Ma, Fen
AU  - Ma F
FAU - Gao, Qiang
AU  - Gao Q
FAU - Zhang, Chengxiao
AU  - Zhang C
FAU - Miao, Wujian
AU  - Miao W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
EDAT- 2010/05/25 06:00
MHDA- 2010/05/25 06:01
CRDT- 2010/05/25 06:00
PHST- 2010/05/25 06:00 [entrez]
PHST- 2010/05/25 06:00 [pubmed]
PHST- 2010/05/25 06:01 [medline]
AID - 10.1021/ac9029289 [doi]
PST - ppublish
SO  - Anal Chem. 2010 Jun 15;82(12):5046-52. doi: 10.1021/ac9029289.