PMID- 20494919
OWN - NLM
STAT- MEDLINE
DCOM- 20101020
LR  - 20161125
IS  - 1469-445X (Electronic)
IS  - 0958-0670 (Linking)
VI  - 95
IP  - 8
DP  - 2010 Aug
TI  - Muscle-specific expression of hypoxia-inducible factor in human skeletal muscle.
PG  - 899-907
LID - 10.1113/expphysiol.2010.052928 [doi]
AB  - Skeletal muscle is well known to exhibit a high degree of plasticity depending on 
      environmental changes, such as various oxygen concentrations. Studies of the 
      oxygen-sensitive subunit alpha of hypoxia-inducible factor-1 (HIF-1) are 
      difficult owing to the large variety of functionally diverse muscle fibres that 
      possess unique patterns of protein and gene expression, producing different 
      capillarization and energy metabolism systems. In this work, we analysed 
      HIF-1alpha mRNA and protein expression related to the fibre-type composition in 
      untrained human skeletal muscle by obtaining muscle biopsies from triceps brachii 
      (characterized by a high proportion of type II fibres), from soleus 
      (characterized by a high proportion of type I fibres) and from vastus lateralis 
      (characterized by an equal proportion of type I and II fibres). The hypothesis 
      was that type I muscle fibres would have lower HIF-1alpha mRNA and protein owing 
      to their higher oxidative capacity. We have shown, in normoxic conditions, a 
      higher HIF-1alpha protein expression in predominantly oxidative muscles than in 
      predominantly glycolytic muscles. However, the HIF-1alpha mRNA expression pattern 
      was not in agreement with the HIF-1alpha protein level. Interestingly, none of 
      the HIF-1alpha target genes, like the most studied angiogenic factor involved in 
      muscle angiogenesis, vascular endothelial growth factor (VEGF), exhibited a 
      muscle fibre-specific-related mRNA expression at rest in normoxia. However, 
      soleus presented a significantly higher VEGF protein content than vastus 
      lateralis and triceps muscle. In conclusion, we have shown that there are 
      muscle-specific differences in HIF-1alpha and VEGF expression within human 
      skeletal muscle at rest in normoxic conditions. Recent results, when combined 
      with the findings described here, support a key role for HIF-1alpha for 
      maintaining muscle homeostasis in non-hypoxic conditions.
FAU - Mounier, Remi
AU  - Mounier R
AD  - Centre of Inflammation and Metabolism, Department of Infectious Diseases, 
      Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
FAU - Pedersen, Bente Klarlund
AU  - Pedersen BK
FAU - Plomgaard, Peter
AU  - Plomgaard P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100521
PL  - England
TA  - Exp Physiol
JT  - Experimental physiology
JID - 9002940
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Adult
MH  - Gene Expression
MH  - Humans
MH  - Hypoxia/metabolism
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*biosynthesis/genetics
MH  - Male
MH  - Muscle Fibers, Fast-Twitch/*metabolism
MH  - Muscle Fibers, Slow-Twitch/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Vascular Endothelial Growth Factor A/genetics
EDAT- 2010/05/25 06:00
MHDA- 2010/10/21 06:00
CRDT- 2010/05/25 06:00
PHST- 2010/05/25 06:00 [entrez]
PHST- 2010/05/25 06:00 [pubmed]
PHST- 2010/10/21 06:00 [medline]
AID - expphysiol.2010.052928 [pii]
AID - 10.1113/expphysiol.2010.052928 [doi]
PST - ppublish
SO  - Exp Physiol. 2010 Aug;95(8):899-907. doi: 10.1113/expphysiol.2010.052928. Epub 
      2010 May 21.