PMID- 20495485
OWN - NLM
STAT- MEDLINE
DCOM- 20110204
LR  - 20211117
IS  - 1529-8809 (Electronic)
IS  - 0022-5282 (Linking)
VI  - 70
IP  - 1
DP  - 2011 Jan
TI  - Inflammatory alterations in a novel combination model of blunt chest trauma and 
      hemorrhagic shock.
PG  - 189-96
LID - 10.1097/TA.0b013e3181d7693c [doi]
AB  - BACKGROUND: Chest trauma frequently occurs in severely injured patients and is 
      often associated with hemorrhagic shock. Immune dysfunction contributes to the 
      adverse outcome of multiple injuries. The aims of this study were to establish a 
      combined model of lung contusion and hemorrhage and to evaluate the 
      cardiopulmonary and immunologic response. METHODS: Male mice were subjected to 
      sham procedure, chest trauma, hemorrhage (35 mm Hg+/-5 mm Hg, 60 minutes), or the 
      combination. Respiratory rate, heart rate, and blood pressure were monitored. 
      Plasma, Kupffer cells, blood monocytes, splenocytes, and splenic macrophages were 
      isolated after 20 hours. Tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-6, 
      10, 12, 18, and macrophage inflammatory protein-2 levels in plasma and culture 
      supernatants were determined. RESULTS: Heart rate and blood pressure dropped in 
      all groups, and after chest trauma and the double hit, these values remained 
      reduced until the end of observation. Blood pressure was lower after the double 
      hit than after the single hits. Plasma and Kupffer cell TNF-alpha concentrations were 
      increased after lung contusion but not further enhanced by subsequent hemorrhage. 
      Peripheral blood mononuclear cell (PBMC) TNF-alpha and IL-6 release were suppressed 
      after the combined insult. IL-18 concentrations were increased in PBMC 
      supernatants after chest trauma and in splenic macrophage supernatants of all 
      groups. CONCLUSIONS: Although physiologic readouts were selectively altered in 
      response to the single or double hits, the combination did not uniformly augment 
      the changes in inflammation. Our results suggest that the leading insult 
      regarding the immunologic response is lung contusion, supporting the concept that 
      lung contusion represents an important prognostic factor in multiple injuries.
FAU - Seitz, Daniel H
AU  - Seitz DH
AD  - Department of Trauma Surgery, Hand, Plastic and Reconstructive Surgery and 
      Division of Surgical Research, University of Ulm, Ulm, Germany.
FAU - Perl, Mario
AU  - Perl M
FAU - Liener, Ulrich C
AU  - Liener UC
FAU - Tauchmann, Bjorn
AU  - Tauchmann B
FAU - Braumuller, Sonja T
AU  - Braumuller ST
FAU - Bruckner, Uwe B
AU  - Bruckner UB
FAU - Gebhard, Florian
AU  - Gebhard F
FAU - Knoferl, Markus W
AU  - Knoferl MW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Trauma
JT  - The Journal of trauma
JID - 0376373
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Interleukins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Blood Pressure/physiology
MH  - Chemokine CXCL2/blood
MH  - *Disease Models, Animal
MH  - Heart Rate/physiology
MH  - Interleukins/blood
MH  - Leukocyte Count
MH  - Macrophages/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Shock, Hemorrhagic/*complications/immunology/pathology/physiopathology
MH  - Spleen/physiopathology
MH  - Thoracic Injuries/*complications/immunology/pathology/physiopathology
MH  - Tumor Necrosis Factor-alpha/blood
MH  - Wounds, Nonpenetrating/*complications/immunology/pathology/physiopathology
EDAT- 2010/05/25 06:00
MHDA- 2011/02/05 06:00
CRDT- 2010/05/25 06:00
PHST- 2010/05/25 06:00 [entrez]
PHST- 2010/05/25 06:00 [pubmed]
PHST- 2011/02/05 06:00 [medline]
AID - 10.1097/TA.0b013e3181d7693c [doi]
PST - ppublish
SO  - J Trauma. 2011 Jan;70(1):189-96. doi: 10.1097/TA.0b013e3181d7693c.