PMID- 20496028
OWN - NLM
STAT- MEDLINE
DCOM- 20101008
LR  - 20160512
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 397
IP  - 6
DP  - 2010 Jul
TI  - Determination of fluoroquinolone residues in poultry muscle in Portugal.
PG  - 2615-21
LID - 10.1007/s00216-010-3819-0 [doi]
AB  - A total of 98 poultry samples, including chicken and turkey muscle, were 
      analysed, using a sensitive and reliable analytical method based on liquid 
      chromatography (LC) with spectrofluorimetric detection, for simultaneous 
      determination of four fluoroquinolone (FQ) antibiotics, namely enrofloxacin 
      (ENRO), ciprofloxacin (CIPRO), norfloxacin (NOR), and sarafloxacin (SARA). The 
      method involved extraction with 0.15 mol L(-1) HCl and clean-up by solid-phase 
      extraction using Oasis HLB cartridges. Chromatographic separation was carried out 
      on a C(18) TSK gel column, in isocratic mode, with 0.025 mol L(-1) H(3)PO(4) 
      solution, adjusted to pH 3.0 with tetrabutylammonium hydroxide-methanol (78:22) 
      as mobile phase. Good linearity over the investigated concentration range was 
      observed, with mean values of correlation coefficients higher than 0.9989 for all 
      the analytes studied. The limits of quantification (LOQ), expressed as the lowest 
      fortification level with acceptable precision were 15 microg kg(-1) for ENRO, 
      CIPRO, and NOR, and 30 microg kg(-1) for SARA; these values are in compliance 
      with requirements for monitoring of maximum residues levels (MRLs). Overall 
      recoveries from spiked samples ranged from 80% to 92% with relative standard 
      deviations (RSD) lower than 6.1%. Of the chicken and turkey samples analysed, 
      44.2% and 37.8%, respectively, were contaminated. The levels found in the 
      analysed poultry samples, collected from markets of Oporto and Coimbra, located 
      in the north and central zones of Portugal, respectively, were lower than 114.2 
      and 87.6 microg kg(-1) in chicken and turkey muscle samples, respectively. One 
      positive chicken sample was contaminated with ENRO at levels higher than the MRL.
FAU - Pena, A
AU  - Pena A
AD  - Group of Health Surveillance, CEF, Faculty of Pharmacy, University of Coimbra, 
      Health Sciences Campus, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal. 
      apena@ff.uc.pt
FAU - Silva, L J G
AU  - Silva LJ
FAU - Pereira, A
AU  - Pereira A
FAU - Meisel, L
AU  - Meisel L
FAU - Lino, C M
AU  - Lino CM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100522
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Fluoroquinolones)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents
MH  - Chromatography, Liquid/methods
MH  - Drug Residues/*analysis
MH  - Fluoroquinolones/*analysis
MH  - Food Contamination/*analysis
MH  - Limit of Detection
MH  - Muscles/chemistry
MH  - Portugal
MH  - *Poultry
MH  - Reproducibility of Results
EDAT- 2010/05/25 06:00
MHDA- 2010/10/12 06:00
CRDT- 2010/05/25 06:00
PHST- 2010/03/31 00:00 [received]
PHST- 2010/05/02 00:00 [accepted]
PHST- 2010/04/22 00:00 [revised]
PHST- 2010/05/25 06:00 [entrez]
PHST- 2010/05/25 06:00 [pubmed]
PHST- 2010/10/12 06:00 [medline]
AID - 10.1007/s00216-010-3819-0 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2010 Jul;397(6):2615-21. doi: 10.1007/s00216-010-3819-0. Epub 
      2010 May 22.