PMID- 20496088
OWN - NLM
STAT- MEDLINE
DCOM- 20100902
LR  - 20100803
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 155
IP  - 8
DP  - 2010 Aug
TI  - Deletion of the cytoplasmic domain of CD163 enhances porcine reproductive and 
      respiratory syndrome virus replication.
PG  - 1319-23
LID - 10.1007/s00705-010-0699-8 [doi]
AB  - The macrophage scavenger receptor CD163 is a major determinant for the entry of 
      porcine reproductive and respiratory syndrome virus (PRRSV). The present study 
      was undertaken to assess the functional significance of the intracellular region 
      of CD163 for PRRSV infection. We stably transfected non-susceptible BHK cells 
      with plasmids encoding full-length and cytoplasmic-domain-deleted (tailless) 
      CD163 and evaluated their permissiveness to PRRSV. The experimental data revealed 
      that the tailless CD163 was not only completely sufficient for converting 
      non-target cells to PRRSV susceptibility, but it also notably enhanced virus 
      replication, with significant increases in viral protein synthesis and progeny 
      release. Taken together, our results suggest that the intracellular domain of 
      CD163 may be associated with an important yet-unknown function during PRRSV 
      infection.
FAU - Lee, Yoo Jin
AU  - Lee YJ
AD  - Department of Microbiology, College of Natural Sciences, Kyungpook National 
      University, Daegu 702-701, South Korea.
FAU - Lee, Changhee
AU  - Lee C
LA  - eng
PT  - Journal Article
DEP - 20100523
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*chemistry/genetics/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/*chemistry/genetics/metabolism
MH  - Cell Line
MH  - Cricetinae
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/*pathogenicity/physiology
MH  - Receptors, Cell Surface/*chemistry/genetics/metabolism
MH  - *Sequence Deletion
MH  - Swine
MH  - Transfection
MH  - *Virus Replication
EDAT- 2010/05/25 06:00
MHDA- 2010/09/03 06:00
CRDT- 2010/05/25 06:00
PHST- 2010/03/30 00:00 [received]
PHST- 2010/05/10 00:00 [accepted]
PHST- 2010/05/25 06:00 [entrez]
PHST- 2010/05/25 06:00 [pubmed]
PHST- 2010/09/03 06:00 [medline]
AID - 10.1007/s00705-010-0699-8 [doi]
PST - ppublish
SO  - Arch Virol. 2010 Aug;155(8):1319-23. doi: 10.1007/s00705-010-0699-8. Epub 2010 
      May 23.