PMID- 20496198
OWN - NLM
STAT- MEDLINE
DCOM- 20100611
LR  - 20171116
IS  - 1477-2213 (Electronic)
IS  - 1028-6020 (Linking)
VI  - 12
IP  - 5
DP  - 2010 May
TI  - Pinocembrin protects the neurovascular unit by reducing inflammation and 
      extracellular proteolysis in MCAO rats.
PG  - 407-18
LID - 10.1080/10286020.2010.485129 [doi]
AB  - The purpose of the present study was to examine the protective action and 
      mechanisms of pinocembrin (1) on the neurovascular unit (NVU) in permanent 
      cerebral ischemic rats. Focal cerebral ischemia was induced by occlusion of 
      middle cerebral artery (MCAO) in rats. Compound 1 (3, 10, or 30 mg/kg) was 
      intravenously injected at 0, 8, 16 h after MCAO. At 24 h of occlusion, 1 
      alleviated neuronal apoptosis, edema of astrocytic end-feet, and the deformation 
      of endothelial cells and capillaries as revealed by the transmission electron 
      microscopy study. To understand the mechanisms of action, the anti-inflammation 
      effect of 1 was examined. Compound 1 reduced the expressions of tumor necrosis 
      factor-alpha, interleukin-1beta, intercellular adhesion molecule-1, vascular cell 
      adhesion molecule-1, inducible NO synthase and aquaporin-4; inhibited the 
      activation of microglias and astrocytes; and downregulated the expression of 
      matrix metalloproteinases (MMPs) in the ischemic brain. The ischemia-induced 
      decreases in mRNA expressions of tight junction constituent proteins, occludin 
      and ZO-1, were also inhibited by 1. These results indicated that 1 can protect 
      the rat brain against ischemia injury by inhibiting the inflammatory cascade, 
      reducing the expression of MMP-9, and preventing the integrity of tight junction. 
      This resulted in the protective action of 1 on the NVU.
FAU - Gao, Mei
AU  - Gao M
AD  - Peking Union Medical College, Institute of Materia Medica and Chinese Academy of 
      Medical Sciences, Beijing, China.
FAU - Zhu, Shen-Yin
AU  - Zhu SY
FAU - Tan, Chu-Bing
AU  - Tan CB
FAU - Xu, Bei
AU  - Xu B
FAU - Zhang, Wen-Cui
AU  - Zhang WC
FAU - Du, Guan-Hua
AU  - Du GH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Asian Nat Prod Res
JT  - Journal of Asian natural products research
JID - 100888334
RN  - 0 (Flavanones)
RN  - 0 (Interleukin-1beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 8T7C8CH791 (pinocembrin)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Brain/drug effects
MH  - Brain Ischemia/chemically induced/*pathology
MH  - Flavanones/pharmacology/*therapeutic use
MH  - Infarction, Middle Cerebral Artery/*chemically induced
MH  - Inflammation/chemically induced/*drug therapy
MH  - Intercellular Adhesion Molecule-1/drug effects
MH  - Interleukin-1beta/drug effects
MH  - RNA, Messenger/drug effects
MH  - Rats
MH  - Tumor Necrosis Factor-alpha/drug effects
MH  - Vascular Cell Adhesion Molecule-1/drug effects
EDAT- 2010/05/25 06:00
MHDA- 2010/06/12 06:00
CRDT- 2010/05/25 06:00
PHST- 2010/05/25 06:00 [entrez]
PHST- 2010/05/25 06:00 [pubmed]
PHST- 2010/06/12 06:00 [medline]
AID - 922439826 [pii]
AID - 10.1080/10286020.2010.485129 [doi]
PST - ppublish
SO  - J Asian Nat Prod Res. 2010 May;12(5):407-18. doi: 10.1080/10286020.2010.485129.