PMID- 20496830
OWN - NLM
STAT- MEDLINE
DCOM- 20100707
LR  - 20151119
IS  - 0005-9366 (Print)
IS  - 0005-9366 (Linking)
VI  - 123
IP  - 5-6
DP  - 2010 May-Jun
TI  - Pharmacological studies of bronchial constriction inhibited by parasympatholytics 
      and cilomilast using equine precision-cut lung slices.
PG  - 229-35
AB  - Equine precision cut lung slices (PCLS) were used to evaluate the ability of 
      several pharmacologic compounds, parasympatholytics atropine, ipratropium, 
      tiotropium and phosphodiesterase (PDE) 4-inhibitor cilomilast, to inhibit 
      methacholine (MCh) induced bronchial constriction. The study war performed using 
      vital lung samples from 20 horses, harvested immediately after euthanasia and 
      lung examination.Tissue samples from the cranial, caudal and accessory lobe were 
      taken for histological examination. Viability in PCLS of 250 +/- 20 microm was 
      confirmed by observation of cilia movement and bronchial contraction in response 
      to the addition of increasing concentrations of MCh (10(-8) to 10(-4) mol/l). 
      Washing in pure RPMI-medium resulted in bronchial reopening. After incubation (20 
      minutes in an RPMI-solution containing cilomilast (10(-5) mol/l), atropine, 
      ipratropium or tiotropium (10(-7) mol/l) each group (randomized, placebo 
      controlled) was treated with MCh again. After reopening the unaffected viability 
      of the PCLS was confirmed by the addition of MCh. EC50 values were calculated 
      from bronchial cross-sections for each contraction. EC50 values significantly 
      increased after atropine, ipratropium and tiotropium addition. No difference was 
      found for cilomilast and in control slices. Statistical analysis showed a large 
      interindividual, but a small intraindivual variability. No correlation was found 
      between the clinical examination, histological findings and PCLS' responsiveness. 
      We were able to confirm Vietmeier's (2007) values for the responsiveness of 
      equine PCLS to MCh and to proof the effect of several new bronchodilatators using 
      a new technique that allows the assessment of several substances in the same 
      horse.
FAU - Barton, Ann Kristin
AU  - Barton AK
AD  - Clinic for Horses, University of Veterinary Medicine Hanover, Foundation, 
      Germany. ann-kristin.barton@tiho-hannover.de
FAU - Niedorf, Frank
AU  - Niedorf F
FAU - Gruber, Achim D
AU  - Gruber AD
FAU - Kietzmann, Manfred
AU  - Kietzmann M
FAU - Ohnesorge, Bernhard
AU  - Ohnesorge B
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Berl Munch Tierarztl Wochenschr
JT  - Berliner und Munchener tierarztliche Wochenschrift
JID - 0003163
RN  - 0 (Bronchodilator Agents)
RN  - 0 (Carboxylic Acids)
RN  - 0 (Cyclohexanecarboxylic Acids)
RN  - 0 (Nitriles)
RN  - 0 (Parasympatholytics)
RN  - 0 (Scopolamine Derivatives)
RN  - 7C0697DR9I (Atropine)
RN  - 8ATB1C1R6X (Cilomilast)
RN  - GR88G0I6UL (Ipratropium)
RN  - XX112XZP0J (Tiotropium Bromide)
SB  - IM
MH  - Animals
MH  - Atropine/pharmacology
MH  - Bronchi/drug effects/*physiology
MH  - Bronchoconstriction/drug effects/*physiology
MH  - Bronchodilator Agents/pharmacology
MH  - Carboxylic Acids/pharmacology
MH  - Cyclohexanecarboxylic Acids
MH  - Euthanasia
MH  - Female
MH  - Horse Diseases/drug therapy/prevention & control
MH  - Horses
MH  - Ipratropium/pharmacology
MH  - Lung/drug effects/*physiology
MH  - Male
MH  - Nitriles/*pharmacology
MH  - Orchiectomy
MH  - Ovariectomy
MH  - Parasympatholytics/*pharmacology
MH  - Scopolamine Derivatives/pharmacology
MH  - Tiotropium Bromide
EDAT- 2010/05/26 06:00
MHDA- 2010/07/08 06:00
CRDT- 2010/05/26 06:00
PHST- 2010/05/26 06:00 [entrez]
PHST- 2010/05/26 06:00 [pubmed]
PHST- 2010/07/08 06:00 [medline]
PST - ppublish
SO  - Berl Munch Tierarztl Wochenschr. 2010 May-Jun;123(5-6):229-35.