PMID- 20496937
OWN - NLM
STAT- MEDLINE
DCOM- 20100806
LR  - 20131121
IS  - 0013-936X (Print)
IS  - 0013-936X (Linking)
VI  - 44
IP  - 12
DP  - 2010 Jun 15
TI  - New sludge pretreatment method to improve methane production in waste activated 
      sludge digestion.
PG  - 4802-8
LID - 10.1021/es1000209 [doi]
AB  - During two-phase sludge anaerobic digestion, sludge is usually hydrolyzed and 
      acidified in the first phase, then methane is produced in the second stage. To 
      get more methane from sludge, most studies in literature focused on the increase 
      of sludge hydrolysis. In this paper a different sludge pretreatment method, i.e., 
      pretreating sludge at pH 10 for 8 d is reported, by which both waste activated 
      sludge hydrolysis and acidification were increased, and the methane production 
      was significantly improved. First, the effect of different sludge pretreatment 
      methods on methane yield was compared. The pH 10 pretreated sludge showed the 
      highest accumulative methane yield (398 mL per g of volatile suspended solids), 
      which was 4.4-, 3.5-, 3.1-, and 2.3-fold of the blank (unpretreated), ultrasonic, 
      thermal, and thermal-alkaline pretreated sludge, respectively. Nevertheless, its 
      total time involved in the first (hydrolysis and acidification) and second 
      (methanogenesis) stages was 17 (8 + 9) d, which was almost the same as other 
      pretreatments. Then, the mechanisms for pH 10 pretreatment significantly 
      improving methane yield were investigated. It was found that pretreating sludge 
      at pH 10 caused the greatest sludge hydrolysis, acidification, soluble C:N and 
      C:P ratios, and Fe(3+) concentration with a suitable short-chain fatty acids 
      composition in the first stage, which resulted in the highest microorganism 
      activity (ATP) and methane production in the second phase. Further investigation 
      on the second phase microorganisms with fluorescence in situ hybridization (FISH) 
      and scanning electron microscopy (SEM) indicated that there were much greater 
      active methanogenesis Archaea when methane was produced with the pH 10 pretreated 
      sludge, and the predominant morphology of the microcolonies suggest a shift to 
      Methanosarcina sp. like.
FAU - Zhang, Dong
AU  - Zhang D
AD  - State Key Laboratory of Pollution Control and Resources Reuse, School of 
      Environmental Science and Engineering, Tongji University, 1239 Siping Road, 
      Shanghai 200092, China.
FAU - Chen, Yinguang
AU  - Chen Y
FAU - Zhao, Yuxiao
AU  - Zhao Y
FAU - Zhu, Xiaoyu
AU  - Zhu X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Fatty Acids)
RN  - 0 (Sewage)
RN  - 0 (Trace Elements)
RN  - 27YLU75U4W (Phosphorus)
RN  - 7440-44-0 (Carbon)
RN  - E1UOL152H7 (Iron)
RN  - N762921K75 (Nitrogen)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Anaerobiosis/drug effects
MH  - Bacteria/cytology/drug effects/metabolism/ultrastructure
MH  - Biodegradation, Environmental/drug effects
MH  - Carbon/analysis
MH  - Fatty Acids/metabolism
MH  - Hydrogen-Ion Concentration/drug effects
MH  - Hydrolysis/drug effects
MH  - In Situ Hybridization, Fluorescence
MH  - Iron/pharmacology
MH  - Methane/*metabolism
MH  - Nitrogen/analysis
MH  - Phosphorus/analysis
MH  - Sewage/*analysis/microbiology
MH  - Time Factors
MH  - Trace Elements/analysis
MH  - Waste Disposal, Fluid/*methods
MH  - Water Purification/*methods
EDAT- 2010/05/26 06:00
MHDA- 2010/08/07 06:00
CRDT- 2010/05/26 06:00
PHST- 2010/05/26 06:00 [entrez]
PHST- 2010/05/26 06:00 [pubmed]
PHST- 2010/08/07 06:00 [medline]
AID - 10.1021/es1000209 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2010 Jun 15;44(12):4802-8. doi: 10.1021/es1000209.