PMID- 20497471
OWN - NLM
STAT- MEDLINE
DCOM- 20101025
LR  - 20181201
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 31
IP  - 11
DP  - 2010 Jun
TI  - GABA receptors and prepulse inhibition of acoustic startle in mice and rats.
PG  - 2053-61
LID - 10.1111/j.1460-9568.2010.07236.x [doi]
AB  - The acoustic startle reflex is strongly inhibited by a moderate-intensity 
      acoustic stimulus that precedes the startling stimulus by roughly 10-1000 ms 
      (prepulse inhibition, PPI). At long interstimulus intervals (ISIs) of 100-1000 
      ms, PPI in rats is reduced by the muscarinic receptor antagonist scopolamine. 
      Here, we studied the role of GABA receptors in PPI at full ISI ranges in both 
      mice and rats. In B6 mice, PPI begins and ends at shorter ISIs (4 and 1000 ms, 
      respectively) than in Wistar rats (8 and 5000 ms). The GABA(A) antagonist 
      bicuculline (1 mg/kg i.p.) reduced PPI at ISIs near the peak of PPI in both rats 
      and mice. The GABA(B) antagonist phaclofen (10 or 30 mg/kg i.p. in rats or mice, 
      respectively) reduced PPI only at long ISIs, similar to the effects of the 
      muscarinic antagonist scopolamine (1 mg/kg i.p.). The effects of phaclofen and 
      scopolamine were additive in rats, suggesting independent effects of GABA(B) and 
      muscarinic receptors. Patch-clamp recordings of startle-mediating PnC (nucleus 
      reticularis pontis caudalis) giant neurons in rat slices show that EPSCs evoked 
      by either trigeminal or auditory fiber stimulation were inhibited by the 
      GABA(A/C) agonist muscimol or the GABA(B) agonist baclofen via postsynaptic 
      mechanisms. Hyperpolarization of PnC neurons by muscimol was reversed with 
      bicuculline, indicating that postsynaptic GABA(A) receptors strongly inhibit PnC 
      giant neurons needed for startle. Therefore, GABA receptors on PnC giant neurons 
      mediate a substantial part of PPI, with GABA(A) receptors contributing at the 
      peak of PPI, and GABA(B) receptors adding to muscarinic effects on PPI at long 
      ISIs.
FAU - Yeomans, John S
AU  - Yeomans JS
AD  - Department of Psychology, Centre for Biological Timing and Cognition, University 
      of Toronto, Toronto, Canada M5S 3G3. yeomans@psych.utoronto.ca
FAU - Bosch, Daniel
AU  - Bosch D
FAU - Alves, Nyresa
AU  - Alves N
FAU - Daros, Alexander
AU  - Daros A
FAU - Ure, Robert J
AU  - Ure RJ
FAU - Schmid, Susanne
AU  - Schmid S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100524
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (GABA Agonists)
RN  - 0 (GABA Antagonists)
RN  - 0 (Muscarinic Antagonists)
RN  - 0 (Receptors, GABA)
RN  - 108351-35-5 (phaclofen)
RN  - 2763-96-4 (Muscimol)
RN  - DL48G20X8X (Scopolamine)
RN  - H789N3FKE8 (Baclofen)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
MH  - *Acoustic Stimulation
MH  - Animals
MH  - Baclofen/analogs & derivatives/pharmacology
MH  - Bicuculline/pharmacology
MH  - GABA Agonists/pharmacology
MH  - GABA Antagonists/pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscarinic Antagonists/pharmacology
MH  - Muscimol/pharmacology
MH  - Neural Inhibition/drug effects/*physiology
MH  - Neurons/drug effects/metabolism
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Receptors, GABA/*metabolism
MH  - Reflex, Startle/drug effects/*physiology
MH  - Scopolamine/pharmacology
MH  - Time Factors
EDAT- 2010/05/26 06:00
MHDA- 2010/10/26 06:00
CRDT- 2010/05/26 06:00
PHST- 2010/05/26 06:00 [entrez]
PHST- 2010/05/26 06:00 [pubmed]
PHST- 2010/10/26 06:00 [medline]
AID - EJN7236 [pii]
AID - 10.1111/j.1460-9568.2010.07236.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2010 Jun;31(11):2053-61. doi: 10.1111/j.1460-9568.2010.07236.x. 
      Epub 2010 May 24.