PMID- 20497712
OWN - NLM
STAT- MEDLINE
DCOM- 20100913
LR  - 20100525
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 123
IP  - 8
DP  - 2010 Apr 20
TI  - Th1 immunity is not required for the effect of lipopolysaccharide exposure on 
      modifying asthmatic responses of mice before sensitization.
PG  - 1047-51
AB  - BACKGROUND: Disequilibrium of Th1/Th2 is known as an important cause of allergic 
      asthma with a biased Th2 type response. It has been shown that lipopolysaccharide 
      (LPS) administration during post-sensitization modified the inflammation of 
      asthma via upregulating the Th1 response that decrease the Th2 immunity. We would 
      like to know if, during pre-sensitization, the elevated Th1 response is necessary 
      for LPS exposure to modify the asthmatic response. METHODS: During pre- or 
      post-sensitization, 40 microg LPS were intraperitoneal injected (i.p.) to 
      asthmatic mice sensitized and challenged by Dermatophagoides farinae (D. 
      farinea). Inflammation was assessed by examining bronchoalveolar lavage fluid 
      (BALF) for the number and identity of cells and by cytokine titers measured by 
      ELISA. Semi-quantified RT-PCR was used to evaluate the level of Toll-like 
      receptor 4 (TLR4) mRNA in dendritic cells (DCs) from bone marrow (BMDCs). 
      RESULTS: These investigations demonstrated that LPS exposure during 
      pre-sensitization inhibited the Th2 cytokine and inflammatory infiltration, the 
      same as with LPS exposure during post-sensitization in allergic asthma mice. 
      Contrary to post-sensitization LPS exposure, the Th1 cytokines were not 
      upregulated by pre-sensitization with LPS. Finally, the study failed to show any 
      significant difference between TLR4 mRNA expressed in BMDCs with the two times of 
      LPS exposure. CONCLUSIONS: Our data suggest that elevated Th1 immunity is not 
      required for the modification of the Th2 response induced by LPS exposure during 
      pre-sensitization in asthmatic mice and that pre-sensitization differs from 
      post-sensitization. Immune modulation with treatment is independent of TLR4 
      expression in BMDCs. This study implicates a potential way to protect from 
      allergic disease and an inflammatory response.
FAU - Wu, Jing
AU  - Wu J
AD  - Department of Immunology and Ecsomatics, Anhui University of Science and 
      Technology, Huainan, China.
FAU - Hu, Dong
AU  - Hu D
FAU - DU, Jiu-wei
AU  - DU JW
FAU - Tao, Xin-rong
AU  - Tao XR
FAU - Qi, Xin-lan
AU  - Qi XL
FAU - Zhang, Rong-bo
AU  - Zhang RB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Animals
MH  - Asthma/chemically induced/*immunology
MH  - Bronchoalveolar Lavage Fluid/immunology
MH  - Cytokines/*immunology/metabolism
MH  - Dendritic Cells/immunology
MH  - Dermatophagoides farinae/immunology
MH  - Female
MH  - Lipopolysaccharides/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Th1 Cells/immunology
MH  - Th2 Cells/*immunology
MH  - Toll-Like Receptor 4/genetics
EDAT- 2010/05/26 06:00
MHDA- 2010/09/14 06:00
CRDT- 2010/05/26 06:00
PHST- 2010/05/26 06:00 [entrez]
PHST- 2010/05/26 06:00 [pubmed]
PHST- 2010/09/14 06:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2010 Apr 20;123(8):1047-51.