PMID- 20497718
OWN - NLM
STAT- MEDLINE
DCOM- 20100913
LR  - 20231213
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 123
IP  - 8
DP  - 2010 Apr 20
TI  - Effect of growth hormone on the immune function of dendritic cells.
PG  - 1078-83
AB  - BACKGROUND: Dendritic cells (DCs) are one of the most important antigen 
      presenting cells in the human body, and DCs at various stages of maturation 
      possess different or even opposite functions. The aim of this study was to 
      investigate the influence of growth hormones on the functional status of cord 
      blood-derived DCs encompassing immunophenotype, ability to excrete interleukin 
      (IL)-12 and provoke autologous leukomonocyte. METHODS: Mononuclear cells were 
      isolated from fresh cord blood, with IL-4 and granulocyte-macrophage 
      colony-stimulating factor (GM-CSF) used to induce and stimulate the mononuclear 
      cells. Growth hormone at different concentrations was used to modify DCs, and 
      then DCs morphology, number and growth status were observed. The immunophenotype 
      of DCs was detected with a flow cytometer. The concentration of IL-12 in the DCs 
      supernatant was determined by enzyme linked immunosorbent assay (ELISA) and DCs 
      functional status was evaluated by autologous mixed lymphocyte reactions. 
      RESULTS: Mononuclear cells from cord blood can be differentiated into DCs by 
      cytokine induction and growth hormone modification. With the increase in growth 
      hormone concentrations (5 - 100 microg/L), the expression of DCs HLA-DR, 
      CD1alpha, CD80 and CD83 were significantly increased (P < 0.05). The ability of 
      DCs to secrete IL-12 was significantly improved (P < 0.05), and the ability of 
      DCs to activate autologous lymphocytes was significantly enhanced (P < 0.05). 
      Pegvisomant was able to ablate the effects of growth hormone on DCs. CONCLUSIONS: 
      Growth hormone may facilitate DCs induction and maturation, and improve the 
      reproductive activity of autologous lymphocytes in a dose-dependent manner. 
      Growth hormone may serve as a factor of modifying DCs to achieving maturity.
FAU - Liu, Qiu-liang
AU  - Liu QL
AD  - Department of Surgery, the First Affiliated Hospital of Zhengzhou University, 
      Henan, China.
FAU - Wang, Yi-sheng
AU  - Wang YS
FAU - Wang, Jia-xiang
AU  - Wang JX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Glycoproteins)
RN  - 187348-17-0 (Interleukin-12)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - 9002-72-6 (Growth Hormone)
SB  - IM
MH  - Antigens, CD/metabolism
MH  - B7-1 Antigen/metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells/*drug effects/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Growth Hormone/*pharmacology
MH  - HLA-DR Antigens/metabolism
MH  - Humans
MH  - Immunoglobulins/metabolism
MH  - Interleukin-12/metabolism
MH  - Membrane Glycoproteins/metabolism
MH  - CD83 Antigen
EDAT- 2010/05/26 06:00
MHDA- 2010/09/14 06:00
CRDT- 2010/05/26 06:00
PHST- 2010/05/26 06:00 [entrez]
PHST- 2010/05/26 06:00 [pubmed]
PHST- 2010/09/14 06:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2010 Apr 20;123(8):1078-83.