PMID- 20498200
OWN - NLM
STAT- MEDLINE
DCOM- 20100727
LR  - 20100622
IS  - 1468-2060 (Electronic)
IS  - 0003-4967 (Linking)
VI  - 69
IP  - 7
DP  - 2010 Jul
TI  - Interleukin 6 is not a crucial regulator in an animal model of tumour necrosis 
      factor-mediated bilateral sacroiliitis.
PG  - 1403-6
LID - 10.1136/ard.2010.129148 [doi]
AB  - OBJECTIVE: To evaluate the role of interleukin 6 (IL-6) in the pathogenesis of 
      bilateral erosive sacroiliitis in human tumour necrosis factor transgenic 
      (hTNFtg) mice, an animal model of ankylosing spondylitis (AS). METHODS: 
      Histological sections of the sacroiliac joints from hTNFtg and IL-6(-/-)hTNFtg 
      mice were evaluated, and wild type and IL-6(-/-)mice served as controls. mRNA 
      levels of inflammation and tissue degradation related genes isolated from 
      sacroiliac joints were also evaluated by quantitative PCR. RESULTS: Severe, 
      erosive bilateral sacroiliitis in 14-week-old hTNFtg animals was accompanied by 
      an upregulation of mRNAs related to tissue inflammation such as matrix 
      metalloproteinase 3 (MMP3), MMP9 and MMP13 or osteoclast activation such as 
      cathepsin K and tartrate-resistant acid phosphatase. In addition, IL-6 was 
      increased in the sera and in the sacroiliac joints of hTNFtg animals. However, 
      high expression of these marker genes in sacroiliac joints from IL-6(-/-)hTNFtg 
      mice was also found. Moreover, absence of IL-6 in these animals did not alter 
      bilateral, erosive sacroiliitis when compared to hTNFtg littermates. CONCLUSIONS: 
      IL-6 is not a crucial regulator in an animal model of TNF-mediated bilateral, 
      erosive sacroiliitis. This finding questions the potential of IL-6 blockade as a 
      new treatment in patients with AS.
FAU - Hayer, Silvia
AU  - Hayer S
AD  - Department of Internal Medicine III, Division of Rheumatology, Medical University 
      of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria.
FAU - Niederreiter, Birgit
AU  - Niederreiter B
FAU - Nagelreiter, Ionela
AU  - Nagelreiter I
FAU - Smolen, Josef
AU  - Smolen J
FAU - Redlich, Kurt
AU  - Redlich K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100524
PL  - England
TA  - Ann Rheum Dis
JT  - Annals of the rheumatic diseases
JID - 0372355
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Arthritis, Experimental/*immunology/pathology
MH  - Female
MH  - Gene Expression Regulation/immunology
MH  - Interleukin-6/deficiency/*immunology
MH  - Male
MH  - Matrix Metalloproteinases/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Osteoclasts/metabolism
MH  - RNA, Messenger/genetics
MH  - Sacroiliac Joint/*immunology/pathology
MH  - Tumor Necrosis Factor-alpha/biosynthesis/genetics/*immunology
EDAT- 2010/05/26 06:00
MHDA- 2010/07/28 06:00
CRDT- 2010/05/26 06:00
PHST- 2010/05/26 06:00 [entrez]
PHST- 2010/05/26 06:00 [pubmed]
PHST- 2010/07/28 06:00 [medline]
AID - ard.2010.129148 [pii]
AID - 10.1136/ard.2010.129148 [doi]
PST - ppublish
SO  - Ann Rheum Dis. 2010 Jul;69(7):1403-6. doi: 10.1136/ard.2010.129148. Epub 2010 May 
      24.