PMID- 20498527
OWN - NLM
STAT- MEDLINE
DCOM- 20100824
LR  - 20131121
IS  - 1361-6528 (Electronic)
IS  - 0957-4484 (Linking)
VI  - 21
IP  - 24
DP  - 2010 Jun 18
TI  - Specific and reversible immobilization of histidine-tagged proteins on 
      functionalized silicon nanowires.
PG  - 245105
LID - 10.1088/0957-4484/21/24/245105 [doi]
AB  - Silicon nanowire (Si NW)-based field effect transistors (FETs) have shown great 
      potential as biosensors (bioFETs) for ultra-sensitive and label-free detection of 
      biomolecular interactions. Their sensitivity depends not only on the device 
      properties, but also on the function of the biological recognition motif attached 
      to the Si NWs. In this study, we show that SiNWs can be chemically functionalized 
      with Ni:NTA motifs, suitable for the specific immobilization of proteins via a 
      short polyhistidine tag (His-tag) at close proximity to the SiNW surface. We 
      demonstrate that the proteins preserve their function upon immobilization onto 
      SiNWs. Importantly, the protein immobilization on the Si NWs is shown to be 
      reversible after addition of EDTA or imidazole, thus allowing the regeneration of 
      the bioFET when needed, such as in the case of proteins having a limited 
      lifetime. We anticipate that our methodology may find a generic use for the 
      development of bioFETs exploiting functional protein assays because of its high 
      compatibility to various types of NWs and proteins.
FAU - Liu, Yi-Chi C
AU  - Liu YC
AD  - Bio-Nanotechnology Laboratory, Department of Neuroscience and Pharmacology & 
      Nano-Science Center, University of Copenhagen, Universitetsparken 5, DK-2100 
      Copenhagen O, Denmark.
FAU - Rieben, Nathalie
AU  - Rieben N
FAU - Iversen, Lars
AU  - Iversen L
FAU - Sorensen, Brian S
AU  - Sorensen BS
FAU - Park, Jiwoong
AU  - Park J
FAU - Nygard, Jesper
AU  - Nygard J
FAU - Martinez, Karen L
AU  - Martinez KL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100525
PL  - England
TA  - Nanotechnology
JT  - Nanotechnology
JID - 101241272
RN  - 0 (Immobilized Proteins)
RN  - 0 (Proteins)
RN  - 4QD397987E (Histidine)
RN  - 7OV03QG267 (Nickel)
RN  - KA90006V9D (Nitrilotriacetic Acid)
RN  - Z4152N8IUI (Silicon)
SB  - IM
MH  - Biosensing Techniques/*instrumentation
MH  - Histidine/*chemistry
MH  - Immobilized Proteins/*chemistry
MH  - Nanowires/*chemistry
MH  - Nickel/chemistry
MH  - Nitrilotriacetic Acid/chemistry
MH  - Proteins/chemistry
MH  - Silicon/*chemistry
EDAT- 2010/05/26 06:00
MHDA- 2010/08/25 06:00
CRDT- 2010/05/26 06:00
PHST- 2010/05/26 06:00 [entrez]
PHST- 2010/05/26 06:00 [pubmed]
PHST- 2010/08/25 06:00 [medline]
AID - S0957-4484(10)39446-3 [pii]
AID - 10.1088/0957-4484/21/24/245105 [doi]
PST - ppublish
SO  - Nanotechnology. 2010 Jun 18;21(24):245105. doi: 10.1088/0957-4484/21/24/245105. 
      Epub 2010 May 25.