PMID- 20501515
OWN - NLM
STAT- MEDLINE
DCOM- 20111021
LR  - 20240109
IS  - 1753-4267 (Electronic)
IS  - 1753-4259 (Linking)
VI  - 17
IP  - 3
DP  - 2011 Feb
TI  - Factors determining the spontaneous activation of splenic dendritic cells in 
      culture.
PG  - 338-52
LID - 10.1177/1753425910371396 [doi]
AB  - Dendritic cells (DCs) serve as a link between the innate and adaptive immune 
      systems. The activation state of DCs is crucial in this role. However, when DCs 
      are isolated from lymphoid tissues, purified and placed in culture they undergo 
      'spontaneous' activation. The basis of this was explored, using up-regulation of 
      DC surface MHC II, CD40, CD80 and CD86 as indicators of DC activation. No 
      evidence was found for DC damage during isolation or for microbial products 
      causing the activation. The culture activation of spleen DCs differed from that 
      of Langerhans cells when released from E-cadherin-mediated adhesions, since 
      E-cadherin was not detected and activation still occurred with beta-catenin null 
      DCs. Much of the activation could be attributed to DC-DC interactions. Although 
      increases in surface MHC II levels occurred under all culture conditions tested, 
      the increase in expression of CD40, CD80 and CD86 was much less under culture 
      conditions where such interactions were minimised. DC-to-DC contact under the 
      artificial conditions of high DC concentration in culture induced the production 
      of soluble factors and these, in turn, induced the up-regulation of 
      co-stimulatory molecules on the DC surface.
FAU - Vremec, David
AU  - Vremec D
AD  - The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, 
      Parkville, Victoria 3050, Australia.
FAU - O'Keeffe, Meredith
AU  - O'Keeffe M
FAU - Wilson, Anne
AU  - Wilson A
FAU - Ferrero, Isabel
AU  - Ferrero I
FAU - Koch, Ute
AU  - Koch U
FAU - Radtke, Freddy
AU  - Radtke F
FAU - Scott, Bernadette
AU  - Scott B
FAU - Hertzog, Paul
AU  - Hertzog P
FAU - Villadangos, Jose
AU  - Villadangos J
FAU - Shortman, Ken
AU  - Shortman K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100525
PL  - United States
TA  - Innate Immun
JT  - Innate immunity
JID - 101469670
RN  - 0 (Antigens, CD)
RN  - 0 (Cadherins)
RN  - 0 (Cytokines)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/*metabolism
MH  - Cadherins/metabolism
MH  - Cell Adhesion
MH  - *Cell Communication/immunology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chimera
MH  - Cytokines/genetics/*metabolism
MH  - Dendritic Cells/immunology/*metabolism/pathology
MH  - Histocompatibility Antigens Class II/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Spleen
MH  - Up-Regulation/immunology
MH  - beta Catenin/genetics
EDAT- 2010/05/27 06:00
MHDA- 2011/10/22 06:00
CRDT- 2010/05/27 06:00
PHST- 2010/05/27 06:00 [entrez]
PHST- 2010/05/27 06:00 [pubmed]
PHST- 2011/10/22 06:00 [medline]
AID - 1753425910371396 [pii]
AID - 10.1177/1753425910371396 [doi]
PST - ppublish
SO  - Innate Immun. 2011 Feb;17(3):338-52. doi: 10.1177/1753425910371396. Epub 2010 May 
      25.