PMID- 20502969
OWN - NLM
STAT- MEDLINE
DCOM- 20110404
LR  - 20211020
IS  - 1574-4647 (Electronic)
IS  - 0161-9152 (Print)
IS  - 0161-9152 (Linking)
VI  - 32
IP  - 4
DP  - 2010 Dec
TI  - The hydrogen sulfide signaling system: changes during aging and the benefits of 
      caloric restriction.
PG  - 467-81
LID - 10.1007/s11357-010-9150-z [doi]
AB  - Hydrogen sulfide gas (H(2)S) is a putative signaling molecule that causes diverse 
      effects in mammalian tissues including relaxation of blood vessels and regulation 
      of perfusion in the liver, but the effects of aging on H(2)S signaling are 
      unknown. Aging has negative impacts on the cardiovascular system. However, the 
      liver is more resilient with age. Caloric restriction (CR) attenuates affects of 
      age in many tissues. We hypothesized that the H(2)S signaling system is 
      negatively affected by age in the vasculature but not in the liver, which is 
      typically more resilient to age, and that a CR diet minimizes the age affect in 
      the vasculature. To investigate this, we determined protein and mRNA expression 
      of the H(2)S-producing enzymes cystathionine gamma-lyase (CSE) and cystathionine 
      beta-synthase (CBS), H(2)S production rates in the aorta and liver, and the 
      contractile response of aortic rings to exogenous H(2)S. Tissue was collected 
      from Fisher 344 x Brown Norway rats from 8-38 months of age, which had been 
      maintained on an ad libitum (AL) or CR diet. The results demonstrate that age and 
      diet have differential effects on the H(2)S signaling system in aorta and liver. 
      The aorta showed a sizeable effect of both age and diet, whereas the liver only 
      showed a sizeable effect of diet. Aortic rings showed increased contractile 
      sensitivity to H(2)S and increased protein expression of CSE and CBS with age, 
      consistent with a decrease in H(2)S concentration with age. CR appears to benefit 
      CSE and CBS protein in both aorta and liver, potentially by reducing oxidative 
      stress and ameliorating the negative effect of age on H(2)S concentration. 
      Therefore, CR may help maintain the H(2)S signaling system during aging.
FAU - Predmore, Benjamin L
AU  - Predmore BL
AD  - Department of Biology, University of Florida, Gainesville, FL 32611, USA.
FAU - Alendy, Maikel J
AU  - Alendy MJ
FAU - Ahmed, Khadija I
AU  - Ahmed KI
FAU - Leeuwenburgh, Christiaan
AU  - Leeuwenburgh C
FAU - Julian, David
AU  - Julian D
LA  - eng
GR  - P30 AG028740/AG/NIA NIH HHS/United States
GR  - R01 AG021042/AG/NIA NIH HHS/United States
GR  - T32 HL083810/HL/NHLBI NIH HHS/United States
GR  - AG21042/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100526
PL  - Netherlands
TA  - Age (Dordr)
JT  - Age (Dordrecht, Netherlands)
JID - 101250497
RN  - 0 (RNA, Messenger)
RN  - EC 4.2.1.22 (Cystathionine beta-Synthase)
RN  - EC 4.4.1.1 (Cystathionine gamma-Lyase)
RN  - YY9FVM7NSN (Hydrogen Sulfide)
SB  - IM
MH  - Aging/*drug effects
MH  - Animals
MH  - Aorta/*drug effects/enzymology
MH  - *Caloric Restriction
MH  - Cystathionine beta-Synthase/genetics/*metabolism
MH  - Cystathionine gamma-Lyase/genetics/*metabolism
MH  - Hydrogen Sulfide/blood/*metabolism
MH  - In Vitro Techniques
MH  - Liver/*drug effects/enzymology
MH  - Oxidative Stress/drug effects
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rats, Inbred F344
MH  - *Signal Transduction
PMC - PMC2980601
EDAT- 2010/05/27 06:00
MHDA- 2011/04/05 06:00
PMCR- 2011/12/01
CRDT- 2010/05/27 06:00
PHST- 2009/07/16 00:00 [received]
PHST- 2010/05/06 00:00 [accepted]
PHST- 2010/05/27 06:00 [entrez]
PHST- 2010/05/27 06:00 [pubmed]
PHST- 2011/04/05 06:00 [medline]
PHST- 2011/12/01 00:00 [pmc-release]
AID - 9150 [pii]
AID - 10.1007/s11357-010-9150-z [doi]
PST - ppublish
SO  - Age (Dordr). 2010 Dec;32(4):467-81. doi: 10.1007/s11357-010-9150-z. Epub 2010 May 
      26.