PMID- 2050297
OWN - NLM
STAT- MEDLINE
DCOM- 19910722
LR  - 20190824
IS  - 0891-5849 (Print)
IS  - 0891-5849 (Linking)
VI  - 10
IP  - 1
DP  - 1991
TI  - Vitamin E, diethylmaleate and bromotrichloromethane interactions in oxidative 
      damage in vivo.
PG  - 51-60
AB  - In vivo interactions of vitamin E with diethylmaleate (DEM) and 
      bromotrichloromethane (CBrCl3) were examined in rats fed a diet either without 
      vitamin E or supplemented with 30 IU dl-alpha-tocopheryl acetate/kg. Groups of 
      rats within each dietary group were given two injections 30 min apart. One group 
      received two injections of the mineral oil carrier. The other groups were 
      injected with either DEM and mineral oil, mineral oil and CBrCl3, or DEM and 
      CBrCl3. The rats were killed 10 min after the second injection. Measurements were 
      made of hepatic GSH, thiobarbituric acid-reactive substances (TBARS) as a lipid 
      peroxidation index, and 11 enzymes as potential markers of oxidant damage. 
      Special focus was placed on reactive cysteine-containing aldehyde dehydrogenase 
      (ALDH). Although dietary vitamin E protected ALDH, the enzyme was highly 
      susceptible to oxidant damage. ALDH activity was correlated with GSH (r = 0.83, p 
      less than 0.001) and there was an inverse relationship between the logarithmic 
      values of ALDH activity and TBARS (r = 0.78, p less than 0.001). Similar results 
      were observed for a number of other enzymes when GSH depletion preceded oxidant 
      treatment. Two-way analysis of variance revealed significant effects of vitamin E 
      and of injection treatments on hepatic GSH. There was a significant interaction 
      between vitamin E and the injection treatments on the activities of five enzymes. 
      The results suggested that vitamin E and GSH functioned together to protect 
      sensitive enzymes against oxidant stress. The sensitive enzymes may be useful 
      markers of hepatic damage in vivo.
FAU - Dillard, C J
AU  - Dillard CJ
AD  - Department of Food Science and Technology, University of California, Davis 95616.
FAU - Hu, M L
AU  - Hu ML
FAU - Tappel, A L
AU  - Tappel AL
LA  - eng
GR  - DK-39225/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
RN  - 0 (Biomarkers)
RN  - 0 (Free Radicals)
RN  - 0 (Maleates)
RN  - 0 (Sulfhydryl Compounds)
RN  - 0 (Thiobarbiturates)
RN  - 1406-18-4 (Vitamin E)
RN  - EC 1.2.1.3 (Aldehyde Dehydrogenase)
RN  - G81WQB56OL (diethyl maleate)
RN  - GAN16C9B8O (Glutathione)
RN  - H4N855PNZ1 (alpha-Tocopherol)
RN  - IKJ30QXM63 (Bromotrichloromethane)
RN  - M1YZW5SS7C (thiobarbituric acid)
RN  - R0ZB2556P8 (Tocopherols)
SB  - IM
MH  - Aldehyde Dehydrogenase/*metabolism
MH  - Animals
MH  - Biomarkers
MH  - Bromotrichloromethane/*pharmacology
MH  - Eating
MH  - Free Radicals
MH  - Glutathione/*metabolism
MH  - Kinetics
MH  - Lipid Peroxidation
MH  - Maleates/*pharmacology
MH  - Mitochondria, Liver/drug effects/enzymology/*metabolism
MH  - Rats
MH  - Sensitivity and Specificity
MH  - Substrate Specificity
MH  - Sulfhydryl Compounds/metabolism
MH  - Thiobarbiturates/metabolism
MH  - Tocopherols
MH  - Vitamin E/*analogs & derivatives/pharmacology
MH  - *alpha-Tocopherol/*analogs & derivatives
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 0891-5849(91)90021-T [pii]
AID - 10.1016/0891-5849(91)90021-t [doi]
PST - ppublish
SO  - Free Radic Biol Med. 1991;10(1):51-60. doi: 10.1016/0891-5849(91)90021-t.