PMID- 20506473
OWN - NLM
STAT- MEDLINE
DCOM- 20100916
LR  - 20220309
IS  - 1096-9861 (Electronic)
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 518
IP  - 14
DP  - 2010 Jul 15
TI  - Cellular and subcellular localization of estrogen and progestin receptor 
      immunoreactivities in the mouse hippocampus.
PG  - 2729-43
LID - 10.1002/cne.22361 [doi]
AB  - Estrogen receptor-alpha (ERalpha), estrogen receptor-beta (ERbeta), and progestin 
      receptor (PR) immunoreactivities are localized to extranuclear sites in the rat 
      hippocampal formation. Because rats and mice respond differently to estradiol 
      treatment at a cellular level, the present study examined the distribution of 
      ovarian hormone receptors in the dorsal hippocampal formation of mice. For this, 
      antibodies to ERalpha, ERbeta, and PR were localized by light and electron 
      immunomicroscopy in male and female mice across the estrous cycle. Light 
      microscopic examination of the mouse hippocampal formation showed sparse nuclear 
      ERalpha and PR immunoreactivity (-ir) most prominently in the CA1 region and 
      diffuse ERbeta-ir primarily in the CA1 pyramidal cell layer as well as in a few 
      interneurons. Ultrastructural analysis additionally revealed discrete 
      extranuclear ERalpha-, ERbeta-, and PR-ir in neuronal and glial profiles 
      throughout the hippocampal formation. Although extranuclear profiles were 
      detected in all animal groups examined, the amount and types of profiles varied 
      with sex and estrous cycle phase. ERalpha-ir was highest in diestrus females, 
      particularly in dendritic spines, axons, and glia. Similarly, ERbeta-ir was 
      highest in estrus and diestrus females, mainly in dendritic spines and glia. 
      Conversely, PR-ir was highest during proestrus, mostly in axons. Except for very 
      low levels of extranuclear ERbeta-ir in mossy fiber terminals in mice, the 
      labeling patterns in the mice for all three antibodies were similar to the 
      ultrastructural labeling found previously in rats, suggesting that regulation of 
      these receptors is well conserved across the two species.
FAU - Mitterling, Katherine L
AU  - Mitterling KL
AD  - Harold and Margaret Milliken Hatch Laboratory of Neuroendocrinology, The 
      Rockefeller University, New York, New York 10065, USA.
FAU - Spencer, Joanna L
AU  - Spencer JL
FAU - Dziedzic, Noelle
AU  - Dziedzic N
FAU - Shenoy, Sushila
AU  - Shenoy S
FAU - McCarthy, Katharine
AU  - McCarthy K
FAU - Waters, Elizabeth M
AU  - Waters EM
FAU - McEwen, Bruce S
AU  - McEwen BS
FAU - Milner, Teresa A
AU  - Milner TA
LA  - eng
GR  - R01 NS007080/NS/NINDS NIH HHS/United States
GR  - GM07739/GM/NIGMS NIH HHS/United States
GR  - R01 DA008259/DA/NIDA NIH HHS/United States
GR  - F30 MH082528/MH/NIMH NIH HHS/United States
GR  - R01 NS007080-41/NS/NINDS NIH HHS/United States
GR  - T32 GM007739/GM/NIGMS NIH HHS/United States
GR  - F30 MH082528-01/MH/NIMH NIH HHS/United States
GR  - R01 DA008259-15/DA/NIDA NIH HHS/United States
GR  - DA08259/DA/NIDA NIH HHS/United States
GR  - HL18974/HL/NHLBI NIH HHS/United States
GR  - T32 DK07313/DK/NIDDK NIH HHS/United States
GR  - NS007080/NS/NINDS NIH HHS/United States
GR  - T32 DK007313-23/DK/NIDDK NIH HHS/United States
GR  - R01 NS007080-42/NS/NINDS NIH HHS/United States
GR  - P01 HL018974/HL/NHLBI NIH HHS/United States
GR  - T32 DK007313/DK/NIDDK NIH HHS/United States
GR  - R01 DA008259-16/DA/NIDA NIH HHS/United States
GR  - P01 HL018974-309002/HL/NHLBI NIH HHS/United States
GR  - R01 NS007080-43/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Receptors, Progesterone)
SB  - IM
MH  - Animals
MH  - CA1 Region, Hippocampal/metabolism
MH  - Estrogen Receptor alpha/*metabolism
MH  - Estrogen Receptor beta/*metabolism
MH  - Estrous Cycle/physiology
MH  - Female
MH  - Hippocampus/*metabolism/ultrastructure
MH  - Interneurons/metabolism/ultrastructure
MH  - Intracellular Space/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neuroglia/metabolism/ultrastructure
MH  - Neurons/metabolism
MH  - Pyramidal Cells/metabolism/ultrastructure
MH  - Receptors, Progesterone/*metabolism
MH  - Sex Characteristics
PMC - PMC2879091
MID - NIHMS199823
EDAT- 2010/05/28 06:00
MHDA- 2010/09/18 06:00
PMCR- 2011/07/15
CRDT- 2010/05/28 06:00
PHST- 2010/05/28 06:00 [entrez]
PHST- 2010/05/28 06:00 [pubmed]
PHST- 2010/09/18 06:00 [medline]
PHST- 2011/07/15 00:00 [pmc-release]
AID - 10.1002/cne.22361 [doi]
PST - ppublish
SO  - J Comp Neurol. 2010 Jul 15;518(14):2729-43. doi: 10.1002/cne.22361.