PMID- 20507062
OWN - NLM
STAT- MEDLINE
DCOM- 20110106
LR  - 20141120
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 44
IP  - 19
DP  - 2010 Oct 1
TI  - Understanding the physiological and molecular mechanism of persistent organic 
      pollutant uptake and detoxification in cucurbit species (zucchini and squash).
PG  - 7295-301
LID - 10.1021/es100116t [doi]
AB  - Cucurbita pepo ssp pepo (zucchini) roots phytoextract significant amounts of 
      persistent organic pollutants (POPs) from soil, followed by effective 
      translocation to aboveground tissues. The closely related C. pepo ssp ovifera 
      (squash) does not have this ability. In a DDE-contaminated field soil, zucchini 
      roots and stems contained 3.6 and 6.6-fold greater contaminant than did squash 
      tissues, respectively, and zucchini phytoextracted 12-times more DDE from soil 
      than squash. In batch hydroponics, squash was significantly more sensitive to DDE 
      (2-20 mg/L) exposure; 4 mg/L DDE significantly reduced squash biomass (14%) 
      whereas for zucchini, biomass reductions were observed at 20 mg/L (20%). PCR 
      select Suppression Subtraction Hybridization was used to identify differentially 
      expressed genes in DDE treated zucchini relative to DDE treated squash or 
      non-treated zucchini. After differential screening to eliminate false positives, 
      unique cDNA clones were sequenced. Out of 40 shoot cDNA sequences, 34 cDNAs have 
      homology to parts of phloem filament protein 1 (PP1). Out of 6 cDNAs from the 
      root tissue, two cDNAs are similar to cytochrome P450 like proteins, and one cDNA 
      matches a putative senescence associated protein. From the DDE exposed zucchini 
      seedlings cDNA library, out of 22 differentially expressed genes, 14 cDNAs were 
      found to have homology with genes involved in abiotic stresses, signaling, lipid 
      metabolism, and photosynthesis. A large number of cDNA sequences were found to 
      encode novel unknown proteins that may be involved in uncharacterized pathways of 
      DDE metabolism in plants. A semiquantitative RT-PCR analysis of isolated genes 
      confirmed up-regulation in response to DDE exposure.
FAU - Chhikara, Sudesh
AU  - Chhikara S
AD  - Department of Plant, Soil and Insect Sciences, University of Massachusetts, 
      Amherst, Massachusetts 01002, USA.
FAU - Paulose, Bibin
AU  - Paulose B
FAU - White, Jason C
AU  - White JC
FAU - Dhankher, Om Parkash
AU  - Dhankher OP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (DNA, Complementary)
RN  - 0 (Soil Pollutants)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Cucurbita/genetics/*metabolism
MH  - DNA, Complementary
MH  - Environmental Restoration and Remediation
MH  - RNA/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Soil Pollutants/*metabolism
EDAT- 2010/05/29 06:00
MHDA- 2011/01/07 06:00
CRDT- 2010/05/29 06:00
PHST- 2010/05/29 06:00 [entrez]
PHST- 2010/05/29 06:00 [pubmed]
PHST- 2011/01/07 06:00 [medline]
AID - 10.1021/es100116t [doi]
PST - ppublish
SO  - Environ Sci Technol. 2010 Oct 1;44(19):7295-301. doi: 10.1021/es100116t.