PMID- 20507871
OWN - NLM
STAT- MEDLINE
DCOM- 20110829
LR  - 20161125
IS  - 1477-0903 (Electronic)
IS  - 0960-3271 (Linking)
VI  - 30
IP  - 5
DP  - 2011 May
TI  - Effects of fumonisin B1 on HLA class I antigen presentation and processing 
      pathway in GES-1 cells in vitro.
PG  - 379-90
LID - 10.1177/0960327110371812 [doi]
AB  - Fumonisin B1 (FB1) is a food-borne mycotoxin produced by genus Fusarium and was 
      classified as possible carcinogen to humans by the International Agency for 
      Research on Cancer (IARC). Human leukocyte antigen (HLA) class I antigen 
      presentation pathway plays an important role in immunosurveillance. Defects in 
      HLA class I antigen presentation pathway can down-regulate the expression of HLA 
      class I antigen on the surface of nucleated cells that will confer a survival 
      advantage to randomly mutant cells and may lead to malignant transformation. In 
      the present study, we analyzed the effects of FB1 on the expression of HLA class 
      I heavy chain (classical HLA-A, -B and -C genes included), beta2-microglobulin 
      (beta2m), LMP2 and TAP1 genes in human gastric epithelial immortalized GES-1 cells 
      in vitro using semi-quantitative Reverse Transcription Polymerase Chain Reaction 
      (RT-PCR), Western blot and immunocytochemical methods in dose- and time-effect 
      studies. Our results revealed that FB1 have an effect on HLA class I antigen 
      presentation pathway via the decreased expression of HLA class I heavy chain 
      and/or defects of LMP2 and TAP1 expression. However, the importance of this 
      effect in carcinogenesis needs further investigation.
FAU - Yao, Z G
AU  - Yao ZG
AD  - Laboratory of Pathology, Hebei Medical University, Shijiazhuang, Hebei, China.
FAU - Zhang, X H
AU  - Zhang XH
FAU - Hua, F
AU  - Hua F
FAU - Wang, J
AU  - Wang J
FAU - Xing, X
AU  - Xing X
FAU - Wang, J L
AU  - Wang JL
FAU - Yan, X
AU  - Yan X
FAU - Xing, L X
AU  - Xing LX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100527
PL  - England
TA  - Hum Exp Toxicol
JT  - Human & experimental toxicology
JID - 9004560
RN  - 0 (Carcinogens, Environmental)
RN  - 0 (Fumonisins)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 3ZZM97XZ32 (fumonisin B1)
SB  - IM
MH  - Antigen Presentation/*drug effects/genetics
MH  - Blotting, Western
MH  - Carcinogens, Environmental/*toxicity
MH  - Cell Culture Techniques
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Epithelial Cells/drug effects/immunology
MH  - Fumonisins/*toxicity
MH  - Gene Expression/*drug effects
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
EDAT- 2010/05/29 06:00
MHDA- 2011/08/30 06:00
CRDT- 2010/05/29 06:00
PHST- 2010/05/29 06:00 [entrez]
PHST- 2010/05/29 06:00 [pubmed]
PHST- 2011/08/30 06:00 [medline]
AID - 0960327110371812 [pii]
AID - 10.1177/0960327110371812 [doi]
PST - ppublish
SO  - Hum Exp Toxicol. 2011 May;30(5):379-90. doi: 10.1177/0960327110371812. Epub 2010 
      May 27.