PMID- 2051025
OWN - NLM
STAT- MEDLINE
DCOM- 19910723
LR  - 20061115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 147
IP  - 1
DP  - 1991 Jul 1
TI  - Experimental onchocerciasis in chimpanzees. Cell-mediated immune responses, and 
      production and effects of IL-1 and IL-2 with Onchocerca volvulus infection.
PG  - 346-53
AB  - Nine of eighteen chimpanzees inoculated with infective third-stage larvae of 
      Onchocerca volvulus developed patent infection with microfilariae in skin 
      biopsies. In all infected chimpanzees the in vitro cellular reactivity to O. 
      volvulus adult worm-derived Ag (OvAg) increased significantly after exposure to 
      third-stage larvae. However, during prepatency the in vitro cellular responses to 
      OvAg decreased gradually in subsequently mf positive (patent) animals, and 
      returned with patency to values not different to those before infection. In 
      non-patent chimpanzees cellular responses remained significantly higher than 
      before infection. Stimulation of PBMC in vitro with bacterial Ag and mitogen did 
      not show any differences between the experimental groups through 20 months p.i. 
      The addition of exogenous IL-2 did not restore the impaired responses of PBMC to 
      OvAg in patent animals. Exogenous IL-2 elicited an additive increase of the 
      cellular response to OvAg in nonpatent, and a mitogenic effect to OvAg in patent 
      animals. Selective depletion of adherent, suppressor/cytotoxic (CD8+), NK cells 
      (CD16+) and the use of autologous serum had no effect on antigenic and mitogenic 
      cellular responsiveness. OvAg-induced IL-2 production decreased after patency, 
      whereas, IL-1 production was significantly greater in both patent and nonpatent 
      than in control chimpanzees. In summary, these data demonstrate that experimental 
      O. volvulus infection in chimpanzees stimulated a substantial cell-mediated 
      immune response. In patent chimpanzees an OvAg-specific cellular 
      hyporesponsiveness occurred before onset of patency, possibly due to decreased 
      IL-2 production and responsiveness.
FAU - Soboslay, P T
AU  - Soboslay PT
AD  - Department of Medicine, Case Western Reserve University, Cleveland, OH 44106.
FAU - Dreweck, C M
AU  - Dreweck CM
FAU - Taylor, H R
AU  - Taylor HR
FAU - Brotman, B
AU  - Brotman B
FAU - Wenk, P
AU  - Wenk P
FAU - Greene, B M
AU  - Greene BM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Bacterial Proteins)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-2)
RN  - 0 (Streptolysins)
RN  - 0 (Tuberculin)
RN  - 0 (streptolysin O)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Bacterial Proteins
MH  - Immunity, Cellular
MH  - Interleukin-1/*pharmacology
MH  - Interleukin-2/*pharmacology
MH  - Lymphocyte Activation
MH  - Onchocerca/*immunology
MH  - Onchocerciasis/*immunology
MH  - Ovalbumin/immunology
MH  - Pan troglodytes
MH  - Streptolysins/pharmacology
MH  - T-Lymphocyte Subsets/*immunology
MH  - Time Factors
MH  - Tuberculin/immunology
EDAT- 1991/07/01 00:00
MHDA- 1991/07/01 00:01
CRDT- 1991/07/01 00:00
PHST- 1991/07/01 00:00 [pubmed]
PHST- 1991/07/01 00:01 [medline]
PHST- 1991/07/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1991 Jul 1;147(1):346-53.