PMID- 20510252
OWN - NLM
STAT- MEDLINE
DCOM- 20110225
LR  - 20151119
IS  - 1095-9319 (Electronic)
IS  - 0026-2862 (Linking)
VI  - 80
IP  - 3
DP  - 2010 Dec
TI  - Unsorted human adipose tissue-derived stem cells promote angiogenesis and 
      myogenesis in murine ischemic hindlimb model.
PG  - 310-6
LID - 10.1016/j.mvr.2010.05.006 [doi]
AB  - We examined the protective effect of unsorted human adipose tissue-derived stem 
      cells (hADSCs) with a short-term culture in endothelial differentiation medium on 
      tissue repair after ischemic injury. hADSCs were isolated from human subcutaneous 
      adipose tissue and cultured in vitro in endothelial differentiation medium for 
      2wks before transplantation. Cultured hADSCs showed a typical mesenchymal stromal 
      cell-like phenotype, positive for endothelial-specific markers including 
      VE-cadherin, Flt-1, eNOS, and vWF but not CD31. Two hours after ligation of the 
      femoral artery and vein, mice were injected with the unselected hADSCs locally 
      near the surgery site and tested for tissue perfusion and repair. Tissue 
      perfusion rates of the ischemic limbs were significantly higher in the group 
      treated with hADSCs compared with those of the control mice as early as 
      post-operative day 3 (median 195.3%/min; interquartile range, 82.0-321.1 vs. 
      median 47.1%/min; interquartile range, 18.0-58.7; p=0.001 by Friedman two-way 
      analysis). Subsequently, the mice treated with hADSC showed better prognosis at 
      4wks after surgery, and the histological analysis revealed increased vascular 
      density and reduced muscle atrophy in the hADSC-transplanted limbs. Moreover, 
      hADSC-treated muscle contained differentiated myocytes positive for human NF-kappaB 
      and myogenin antigen. These results collectively indicate that unsorted hADSCs 
      after a 2-wk-in vitro culture have a therapeutic potential in ischemic tissue 
      injury via inducing both angiogenesis and myogenesis.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Kang, Yujung
AU  - Kang Y
AD  - Department of Bio and Brain Engineering, KAIST, Republic of Korea.
FAU - Park, Chan
AU  - Park C
FAU - Kim, Daham
AU  - Kim D
FAU - Seong, Chu-Myong
AU  - Seong CM
FAU - Kwon, Kihwan
AU  - Kwon K
FAU - Choi, Chulhee
AU  - Choi C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100525
PL  - United States
TA  - Microvasc Res
JT  - Microvascular research
JID - 0165035
RN  - 0 (Biomarkers)
RN  - 0 (Culture Media)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Culture Media
MH  - Disease Models, Animal
MH  - Endothelial Cells/metabolism/*transplantation
MH  - Female
MH  - Hindlimb
MH  - Humans
MH  - Ischemia/metabolism/pathology/physiopathology/*surgery
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Nude
MH  - *Muscle Development
MH  - Muscle, Skeletal/*blood supply/metabolism/pathology/*physiopathology
MH  - Muscular Atrophy/pathology/prevention & control
MH  - Necrosis
MH  - *Neovascularization, Physiologic
MH  - Perfusion Imaging
MH  - Phenotype
MH  - *Stem Cell Transplantation
MH  - Subcutaneous Fat, Abdominal/*cytology/metabolism
MH  - Time Factors
EDAT- 2010/06/01 06:00
MHDA- 2011/02/26 06:00
CRDT- 2010/06/01 06:00
PHST- 2010/04/07 00:00 [received]
PHST- 2010/05/11 00:00 [revised]
PHST- 2010/05/14 00:00 [accepted]
PHST- 2010/06/01 06:00 [entrez]
PHST- 2010/06/01 06:00 [pubmed]
PHST- 2011/02/26 06:00 [medline]
AID - S0026-2862(10)00127-5 [pii]
AID - 10.1016/j.mvr.2010.05.006 [doi]
PST - ppublish
SO  - Microvasc Res. 2010 Dec;80(3):310-6. doi: 10.1016/j.mvr.2010.05.006. Epub 2010 
      May 25.