PMID- 20510320
OWN - NLM
STAT- MEDLINE
DCOM- 20110131
LR  - 20111117
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 71
IP  - 9
DP  - 2010 Sep
TI  - Specific "intra-allele" and "intra-broad antigen" human leukocyte antigen 
      alloantibodies in kidney graft transplantation.
PG  - 857-60
LID - 10.1016/j.humimm.2010.05.018 [doi]
AB  - Human leukocyte antigen (HLA) antibodies are epitope specific and not antigen 
      specific. This work presents a case of intra-allele (IA) sensitization. A 
      40-year-old-man underwent transplantion with identical "broad" DR. He was 
      apparently not sensitized to HLA antigens by complement-dependent cytotoxicity 
      (CDC), with one previous transplantation 15 years previously. In 
      post-transplantation monitoring, we detected an "intra-broad antigen" (IBA) 
      anti-DRB1*13 DSA by Luminex. We performed post-transplantation B-cell 
      cross-matching (CM) by CDC, this being completely negative. We detected 
      allele-specific antibodies by single antigens (SA), anti-DRB1*1303 (IBA), 
      -DQB1*0301 (IA), -DRB1*1101, -DRB3*0101, anti-DPB1*0202, and anti-DRB1*0103. 
      These antibodies originated from the first transplantation, HLA-DR6+ homozygous 
      and serologically broad matched, but retrospectively typed as DRB1*1401, *1303; 
      DRB3*0101, *0202; DQB1*0301, *0503; DPB1*0401, *0202 (mismatches in italics). 
      However the second donor was DRB1*1301, *1401 (DR6+ homozygous); DRB3*0202; 
      DQB1*0603, *0503; DPB1*0401 (mismatches in italic). Therefore, the stronger 
      antibodies generated in the first transplantion (anti-DRB1*1303 and -DQB1*0301) 
      were not specific for the specific subtypes (DRB1*1301 and -DQB1*0603) on the 
      second transplantation. Finally, it was possible to exactly define the potential 
      immunizing epitopes the recognition of which determined antibody production. 
      Therefore, our patient had low titers of pretransplantation IBA and IA antibodies 
      that were not prospectively detected by CDC. Post-transplantation with Luminex, 
      we detected these alloantibodies, but as they were not IA and IBA DSA, they did 
      not cause allograft injury.
CI  - Copyright 2010 American Society for Histocompatibility and Immunogenetics. 
      Published by Elsevier Inc. All rights reserved.
FAU - Muro, Manuel
AU  - Muro M
AD  - Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas, 
      Murcia, Spain. manuel.muro@carm.es
FAU - Gonzalez-Soriano, Maria J
AU  - Gonzalez-Soriano MJ
FAU - Salgado, Gema
AU  - Salgado G
FAU - Lopez, Ruth
AU  - Lopez R
FAU - Boix, Francisco
AU  - Boix F
FAU - Lopez, Manuela
AU  - Lopez M
FAU - Campillo, Jose A
AU  - Campillo JA
FAU - Martinez, Pedro
AU  - Martinez P
FAU - Botella, Carmen
AU  - Botella C
FAU - Gimeno, Luisa
AU  - Gimeno L
FAU - Minguela, Alfredo
AU  - Minguela A
FAU - Alvarez-Lopez, Maria R
AU  - Alvarez-Lopez MR
FAU - Llorente, Santiago
AU  - Llorente S
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100525
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-DP Antigens)
RN  - 0 (HLA-DP beta-Chains)
RN  - 0 (HLA-DPB1 antigen)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQB1 antigen)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (HLA-DRB1*13 antigen)
RN  - 0 (HLA-DRB3 Chains)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence/genetics
MH  - Amino Acid Substitution/genetics/immunology
MH  - B-Lymphocytes/immunology
MH  - Computational Biology
MH  - Cytotoxicity Tests, Immunologic
MH  - Epitopes, B-Lymphocyte/genetics/immunology
MH  - Graft Rejection/immunology
MH  - Graft Survival/immunology
MH  - HLA Antigens/genetics/*immunology
MH  - HLA-DP Antigens/genetics/immunology
MH  - HLA-DP beta-Chains
MH  - HLA-DQ Antigens/genetics/immunology
MH  - HLA-DQ beta-Chains
MH  - HLA-DR Antigens/genetics/immunology
MH  - HLA-DRB1 Chains
MH  - HLA-DRB3 Chains
MH  - Histocompatibility Antigens Class I/genetics
MH  - Histocompatibility Antigens Class II/genetics/immunology
MH  - Histocompatibility Testing
MH  - Humans
MH  - Isoantibodies/blood/*immunology
MH  - Kidney Transplantation/*immunology
MH  - Male
MH  - Molecular Sequence Data
MH  - Polymorphism, Genetic/genetics/immunology
EDAT- 2010/06/01 06:00
MHDA- 2011/02/01 06:00
CRDT- 2010/06/01 06:00
PHST- 2009/12/30 00:00 [received]
PHST- 2010/03/16 00:00 [revised]
PHST- 2010/05/19 00:00 [accepted]
PHST- 2010/06/01 06:00 [entrez]
PHST- 2010/06/01 06:00 [pubmed]
PHST- 2011/02/01 06:00 [medline]
AID - S0198-8859(10)00129-1 [pii]
AID - 10.1016/j.humimm.2010.05.018 [doi]
PST - ppublish
SO  - Hum Immunol. 2010 Sep;71(9):857-60. doi: 10.1016/j.humimm.2010.05.018. Epub 2010 
      May 25.