PMID- 20511709
OWN - NLM
STAT- MEDLINE
DCOM- 20100922
LR  - 20220318
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 25
IP  - 6
DP  - 2010
TI  - Stimulation of mouse dendritic cells by Gum Arabic.
PG  - 641-8
LID - 10.1159/000315083 [doi]
AB  - Gum Arabic (GA), a nonabsorbable nutrient manufactured from the exudate of Acacia 
      senegal, is composed of a complex polysaccharide. GA is used by the 
      pharmaceutical and food industry as an emulsifier but may, at an appropriate 
      dosage, modify intestinal transport. Dendritic cells (DCs) can protrude between 
      epithelial cells and sense the composition of the lumen. As DCs are stimulated by 
      bacterial polysaccharides, we hypothesized that GA may similarly stimulate DCs. 
      To test that hypothesis, mouse DCs were treated with either LPS or GA and 
      expression of maturation markers, phagocytotic activity, cytokine production and 
      ability to stimulate CD4(+) T cells in allogenic mixed leukocyte reaction 
      (allo-MLR) was analyzed. As a result both LPS and GA increased the percentage of 
      CD11c(+)CD86(+), CD11c(+)MHCII(+), CD11c(+)CD40(+), CD54-expressing DCs and 
      decreased their phagocytic activity. Both LPS and GA stimulated the production of 
      IL-6, IL-10, IL12p70 and TNFalpha in a p38- and/or ERK-dependent manner. GA 
      treatment led to an enhanced IL-10 secretion, whereas LPS was more effective on 
      IL-6 and IL-12p70 production. Both LPS- and GA-stimulated DCs enhanced CD4(+) T 
      cell proliferation but the profile of cytokines produced in allo-MLR was 
      different. High levels of IL-10 and IL-6 were observed in the presence of 
      GA-treated DCs, whereas IFN-gamma and IL-12p70 production was similar with LPS- 
      or GA-treated DCs. LPS upregulated p38 and transiently ERK1/2, while GA led to 
      more sustained activation of ERK1/2, only. In conclusion, the observations reveal 
      a powerful immunomodulatory effect of GA.
CI  - Copyright 2010 S. Karger AG, Basel.
FAU - Xuan, Nguyen Thi
AU  - Xuan NT
AD  - Department of Physiology, University of Tubingen, 72076 Tubingen, Germany.
FAU - Shumilina, Ekaterina
AU  - Shumilina E
FAU - Nasir, Omaima
AU  - Nasir O
FAU - Bobbala, Diwakar
AU  - Bobbala D
FAU - Gotz, Friedrich
AU  - Gotz F
FAU - Lang, Florian
AU  - Lang F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100518
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 9000-01-5 (Gum Arabic)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Acacia/chemistry
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cytokines/*immunology
MH  - Dendritic Cells/*cytology/immunology
MH  - Gum Arabic/*metabolism
MH  - Leukocytes/immunology
MH  - Lipopolysaccharides/*immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinase 1/genetics
MH  - Mitogen-Activated Protein Kinase 3/genetics
MH  - *Phagocytosis
MH  - Up-Regulation
EDAT- 2010/06/01 06:00
MHDA- 2010/09/24 06:00
CRDT- 2010/06/01 06:00
PHST- 2010/03/02 00:00 [accepted]
PHST- 2010/06/01 06:00 [entrez]
PHST- 2010/06/01 06:00 [pubmed]
PHST- 2010/09/24 06:00 [medline]
AID - 000315083 [pii]
AID - 10.1159/000315083 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2010;25(6):641-8. doi: 10.1159/000315083. Epub 2010 May 18.