PMID- 20512480
OWN - NLM
STAT- MEDLINE
DCOM- 20100611
LR  - 20211020
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 31
IP  - 3
DP  - 2010 Jun
TI  - MET overexpressing chordomas frequently exhibit polysomy of chromosome 7 but no 
      MET activation through sarcoma-specific gene fusions.
PG  - 157-63
LID - 10.1007/s13277-010-0021-0 [doi]
AB  - Overexpression of MET and polysomy 7 was formerly demonstrated in chordomas. We 
      investigated mesenchymal-epithelial transition factor (MET) protein expression 
      and copy numbers of chromosome 7 in human chordomas. Furthermore, tumors were 
      screened for gene fusions (PAX3-FKHR, ASPL-TFE3, and SYT-SSX) previously shown to 
      be associated with MET activation in sarcomas. Tissue microarrays (TMAs) were 
      constructed from 66 chordoma samples. MET protein expression was assessed by 
      immunohistochemistry using an immunoreactive score (IRS, scores 0-12). 
      fluorescence in situ hybridization (FISH) with a dual-color DNA probe (7q31) for 
      MET amplification was performed on TMA sections and RT-PCR for PAX3-FKHR, 
      ASPL-TFE3 (type 1 + 2), and SYT-SSX (type 1 + 2) gene fusions on punch biopsies. 
      All tumors (n = 66) expressed MET protein. FISH analysis of 33 tumors lacked MET 
      gene amplification but showed polysomy of chromosome 7 in 15 (45.5%) tumors (13 
      low and two high polysomies). Although, polysomy 7 showed an increasing incidence 
      with escalating MET IRS, this finding was not statistically significant. 
      PAX3-FKHR, ASPL-TFE3, or SYT-SSX gene fusions were not demonstrable (n = 52). We 
      found MET protein expression in all chordomas. A clear influence of polysomy 7 on 
      MET protein expression could not be statistically demonstrated for this cohort. 
      Moreover, gene fusions with the ability to cause MET overexpression do not occur 
      in chordomas.
FAU - Grabellus, Florian
AU  - Grabellus F
AD  - Institute of Pathology and Neuropathology, University Hospital of Essen, 
      University of Duisburg-Essen, Hufelandstrasse 55, 45122, Essen, Germany. 
      florian.grabellus@uk-essen.de
FAU - Konik, Margarethe J
AU  - Konik MJ
FAU - Worm, Karl
AU  - Worm K
FAU - Sheu, Sien-Yi
AU  - Sheu SY
FAU - van de Nes, Johannes A P
AU  - van de Nes JA
FAU - Bauer, Sebastian
AU  - Bauer S
FAU - Paulus, Werner
AU  - Paulus W
FAU - Egensperger, Rupert
AU  - Egensperger R
FAU - Schmid, Kurt W
AU  - Schmid KW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100306
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Aneuploidy
MH  - Child
MH  - Chordoma/*genetics/metabolism
MH  - Chromosomes, Human, Pair 7/*genetics
MH  - Female
MH  - *Gene Fusion
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Proteins c-met/*metabolism
MH  - Sarcoma/genetics/metabolism
MH  - Young Adult
EDAT- 2010/06/01 06:00
MHDA- 2010/06/12 06:00
CRDT- 2010/06/01 06:00
PHST- 2009/07/24 00:00 [received]
PHST- 2009/10/08 00:00 [accepted]
PHST- 2010/06/01 06:00 [entrez]
PHST- 2010/06/01 06:00 [pubmed]
PHST- 2010/06/12 06:00 [medline]
AID - 10.1007/s13277-010-0021-0 [doi]
PST - ppublish
SO  - Tumour Biol. 2010 Jun;31(3):157-63. doi: 10.1007/s13277-010-0021-0. Epub 2010 Mar 
      6.