PMID- 20512733
OWN - NLM
STAT- MEDLINE
DCOM- 20111006
LR  - 20240109
IS  - 1532-4109 (Electronic)
IS  - 0360-1234 (Linking)
VI  - 45
IP  - 5
DP  - 2010 Jul
TI  - Influence of soil fumigation by methyl bromide and methyl iodide on rhizosphere 
      and phyllosphere microbial community structure.
PG  - 427-36
LID - 10.1080/03601231003800131 [doi]
AB  - Rhizosphere and phyllosphere microbial communities were evaluated on roots and 
      leaves of growth chamber-grown lettuce (Lactuca sativa (L.) cv. Green Forest) 
      plants by culture-dependent and -independent methods after soil fumigation. 
      Denaturing gradient gel electrophoresis (DGGE) with 16S rRNA primers followed by 
      cloning and sequencing was used to identify major rRNA bands from the rhizosphere 
      and phyllosphere. Three weeks after fumigation, there were no differences (P = 
      0.16) in rhizosphere microbial communities between the fumigated treatments and 
      the control. The same effect was observed during week seven after fumigation 
      (P=0.49). Also, no significant differences (P=0.49) were found in the 
      phyllosphere microbial communities between the fumigated treatments and the 
      control during the growth period of the plant. A majority of the bands in the 
      rhizosphere were related to known bacterial sequences with a 96 to 100 % sequence 
      similarity. Some of the derived sequences were related to Pseudomonas syringae 
      pv. tomato DC300 and Bradyrhizobium japonicum USDA 110. A total of 23 isolates 
      were identified from leaf surface by both culture-dependent and independent 
      methods, and only Photorhabdus luminescens was found on leaf surface using both 
      techniques. All the Biolog isolates from phyllosphere were from the 
      Proteobacteria phylum compared to the culture-independent bands from the leaves 
      that were from different bacterial phyla. Based on our data, methyl bromide 
      (MeBr) and methyl iodide (MeI) did not have any significant negative effects on 
      rhizosphere and phyllosphere microbial communities throughout the growing period 
      of lettuce.
FAU - Ibekwe, A M
AU  - Ibekwe AM
AD  - USDA-ARS, U.S. Salinity Laboratory, Riverside, California 92507, USA. 
      Mark.Ibekwe@ars.usda.gov
FAU - Papiernik, S K
AU  - Papiernik SK
FAU - Yang, C-H
AU  - Yang CH
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Environ Sci Health B
JT  - Journal of environmental science and health. Part. B, Pesticides, food 
      contaminants, and agricultural wastes
JID - 7607167
RN  - 0 (DNA, Bacterial)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 0 (Hydrocarbons, Iodinated)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil Pollutants)
RN  - 9V42E1Z7B6 (methyl bromide)
RN  - DAT010ZJSR (methyl iodide)
SB  - IM
MH  - DNA, Bacterial/analysis
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Environmental Exposure
MH  - *Fumigation
MH  - Hepatophyta/drug effects/genetics/growth & development
MH  - Hydrocarbons, Brominated/*pharmacology
MH  - Hydrocarbons, Iodinated/*pharmacology
MH  - Lactuca/*drug effects/*microbiology
MH  - Plant Leaves/drug effects/microbiology
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rhizobium/drug effects/genetics/growth & development
MH  - *Rhizosphere
MH  - Seasons
MH  - *Soil Microbiology
MH  - Soil Pollutants/pharmacology
MH  - Time Factors
EDAT- 2010/06/01 06:00
MHDA- 2011/10/07 06:00
CRDT- 2010/06/01 06:00
PHST- 2010/06/01 06:00 [entrez]
PHST- 2010/06/01 06:00 [pubmed]
PHST- 2011/10/07 06:00 [medline]
AID - 922593825 [pii]
AID - 10.1080/03601231003800131 [doi]
PST - ppublish
SO  - J Environ Sci Health B. 2010 Jul;45(5):427-36. doi: 10.1080/03601231003800131.