PMID- 20514487
OWN - NLM
STAT- MEDLINE
DCOM- 20110121
LR  - 20211020
IS  - 1432-136X (Electronic)
IS  - 0174-1578 (Linking)
VI  - 180
IP  - 7
DP  - 2010 Oct
TI  - Metabolic and molecular stress responses of gilthead seam bream Sparus aurata 
      during exposure to low ambient temperature: an analysis of mechanisms underlying 
      the winter syndrome.
PG  - 1005-18
LID - 10.1007/s00360-010-0481-y [doi]
AB  - The winter syndrome in the gilthead sea bream Sparus aurata indicates that the 
      species is exposed to critically low temperatures in Mediterranean aquaculture in 
      winter. The present study of metabolic patterns and molecular stress responses 
      during cold exposure was carried out to investigate this "disease", in light of 
      the recent concept of oxygen and capacity limited thermal tolerance. The 
      metabolic profile of fuel oxidation was examined by determining the activities of 
      the enzymes hexokinase (HK), aldolase (Ald), pyruvate kinase (PK), L-lactate 
      dehydrogenase (L-LDH), citrate synthase (CS), malate dehydrogenase (MDH) and 
      3-hydroxyacyl CoA dehydrogenase (HOAD) in heart, red and white muscle after 
      exposure to temperatures of 10, 14 and 18 degrees C. Especially, the increase in LDH 
      activity combined with the accumulation of L-lactate in tissues indicates that 
      temperatures below 14 degrees C are critical for Sparus aurata and stimulate the 
      anaerobic component of metabolism. Increase in the activity of HOAD suggests that 
      oxidation of free fatty acids might contribute to ATP turnover at low 
      temperatures. The expression of Hsp70 and Hsp90 in all tissues examined revealed 
      a cellular stress response during cooling below 18 degrees C. In the light of winter 
      temperatures in S. aurata cultures around 10 degrees C, our data suggest that the fish 
      are exposed to stressful conditions at the low end of their thermal tolerance 
      window. These conditions likely impair the aerobic capacity of the fish, 
      compromise the rates of growth and reproduction and may contribute to elicit 
      pathological conditions.
FAU - Kyprianou, Themis-Dimitrios
AU  - Kyprianou TD
AD  - Laboratory of Animal Physiology, Department of Zoology, Faculty of Sciences, 
      School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, 
      Greece.
FAU - Portner, Hans O
AU  - Portner HO
FAU - Anestis, Andreas
AU  - Anestis A
FAU - Kostoglou, Basile
AU  - Kostoglou B
FAU - Feidantsis, Konstantinos
AU  - Feidantsis K
FAU - Michaelidis, Basile
AU  - Michaelidis B
LA  - eng
PT  - Journal Article
DEP - 20100601
PL  - Germany
TA  - J Comp Physiol B
JT  - Journal of comparative physiology. B, Biochemical, systemic, and environmental 
      physiology
JID - 8413200
RN  - 0 (Blood Glucose)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (HSP90 Heat-Shock Proteins)
RN  - 0 (Triglycerides)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Acclimatization/*physiology
MH  - Animals
MH  - Aquaculture
MH  - Blood Glucose/analysis
MH  - Cold Temperature/adverse effects
MH  - *Energy Metabolism
MH  - HSP70 Heat-Shock Proteins/metabolism
MH  - HSP90 Heat-Shock Proteins/metabolism
MH  - Lactic Acid/blood/metabolism
MH  - Liver/enzymology/metabolism
MH  - Muscle Fibers, Skeletal/enzymology/metabolism
MH  - Myocardium/enzymology/metabolism
MH  - Sea Bream/blood/*physiology
MH  - *Seasons
MH  - Seawater
MH  - Time Factors
MH  - Triglycerides/blood
EDAT- 2010/06/02 06:00
MHDA- 2011/01/22 06:00
CRDT- 2010/06/02 06:00
PHST- 2010/01/15 00:00 [received]
PHST- 2010/05/17 00:00 [accepted]
PHST- 2010/05/03 00:00 [revised]
PHST- 2010/06/02 06:00 [entrez]
PHST- 2010/06/02 06:00 [pubmed]
PHST- 2011/01/22 06:00 [medline]
AID - 10.1007/s00360-010-0481-y [doi]
PST - ppublish
SO  - J Comp Physiol B. 2010 Oct;180(7):1005-18. doi: 10.1007/s00360-010-0481-y. Epub 
      2010 Jun 1.