PMID- 20514989
OWN - NLM
STAT- MEDLINE
DCOM- 20100708
LR  - 20220317
IS  - 0895-3988 (Print)
IS  - 0895-3988 (Linking)
VI  - 23
IP  - 2
DP  - 2010 Apr
TI  - Association of TNF-alpha-238G/A and 308 G/A gene polymorphisms with pulmonary 
      tuberculosis among patients with coal worker's pneumoconiosis.
PG  - 137-45
LID - 10.1016/S0895-3988(10)60043-8 [doi]
AB  - OBJECTIVES: Tumor necrosis factor-alpha (TNF-alpha) may play an important role in 
      host's immune response to mycobacterium tuberculosis (M. tuberculosis) infection. 
      This study was to investigate the association of TNF-alpha gene polymorphism with 
      pulmonary tuberculosis (TB) among patients with coal worker's pneumoconiosis 
      (CWP). METHODS: A case-control study was conducted in 113 patients with confirmed 
      CWP complicated with pulmonary TB and 113 non-TB controls with CWP. They were 
      matched in gender, age, job, and stage of pneumoconiosis. All participants were 
      interviewed with questionnaires and their blood specimens were collected for 
      genetic determination with informed consent. The TNF-alpha gene polymorphism was 
      determined with polymerase chain reaction of restriction fragment length 
      polymorphism (PCR-RFLP). Frequency of genotypes was assessed for Hardy-Weinberg 
      equilibrium by chi-square test or Fisher's exact probability. Factors influencing 
      the association of individual susceptibility with pulmonary TB were evaluated 
      with logistic regression analysis. Gene-environment interaction was evaluated by 
      a multiplicative model with combined OR. All data were analyzed using SAS version 
      8.2 software. RESULTS: No significant difference in frequency of the 
      TNF-alpha-308 genotype was found between CWP complicated with pulmonary TB and 
      non-TB controls (chi2 = 5.44, P = 0.07). But difference in frequency of the 
      TNF-alpha-308 A allele was identified between them (chi2 = 5.14, P = 0.02). No 
      significant difference in frequencies of the TNF-alpha-238 genotype and allele (P 
      = 0.23 and P = 0.09, respectively) was found between cases and controls either, 
      with combined (GG and AA) OR of 3.96 (95% confidence interval of 1.30-12.09) at 
      the -308 locus of the TNF-alpha gene, as compared to combination of the 
      TNF-alpha-238 GG and TNF-alpha-308 GG genotypes. Multivariate-adjusted odds ratio 
      of the TNF-alpha-238 GG and TNF-alpha-308 GA genotypes was 1.98 (95% CI of 
      1.06-3.71) for risk for pulmonary TB in patients with CWP. There was a synergic 
      interaction between the TNF-alpha-308 GG genotype and body mass index (OR = 
      4.92), as well as an interaction between the TNF-alpha-308 GG genotype and 
      history of BCG immunization or history of TB exposure. And, the interaction of 
      the TNF-alpha-238 GG genotype and history of BCG immunization or TB exposure with 
      risk for pulmonary TB in them was also indicated. CONCLUSIONS: TNF-alpha-308 A 
      allele is associated with an elevated risk for pulmonary TB, whereas 
      TNF-alpha-238 A allele was otherwise.
FAU - Fan, Hong-Min
AU  - Fan HM
AD  - School of Basic Medicine, Peking Union Medical College, Institute of Basic 
      Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China.
FAU - Wang, Zhuo
AU  - Wang Z
FAU - Feng, Fu-Min
AU  - Feng FM
FAU - Zhang, Kong-Lai
AU  - Zhang KL
FAU - Yuan, Ju-Xiang
AU  - Yuan JX
FAU - Sui, Hong
AU  - Sui H
FAU - Qiu, Hong-Yan
AU  - Qiu HY
FAU - Liu, Li-Hua
AU  - Liu LH
FAU - Deng, Xiao-Juan
AU  - Deng XJ
FAU - Ren, Jing-Xue
AU  - Ren JX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Biomed Environ Sci
JT  - Biomedical and environmental sciences : BES
JID - 8909524
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Anthracosis/*complications
MH  - Case-Control Studies
MH  - Environmental Exposure
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - Logistic Models
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Single Nucleotide
MH  - Promoter Regions, Genetic
MH  - Tuberculosis, Pulmonary/*genetics
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2010/06/03 06:00
MHDA- 2010/07/09 06:00
CRDT- 2010/06/03 06:00
PHST- 2010/06/03 06:00 [entrez]
PHST- 2010/06/03 06:00 [pubmed]
PHST- 2010/07/09 06:00 [medline]
AID - S0895-3988(10)60043-8 [pii]
AID - 10.1016/S0895-3988(10)60043-8 [doi]
PST - ppublish
SO  - Biomed Environ Sci. 2010 Apr;23(2):137-45. doi: 10.1016/S0895-3988(10)60043-8.