PMID- 2051523
OWN - NLM
STAT- MEDLINE
DCOM- 19910723
LR  - 20121115
IS  - 0022-5304 (Print)
IS  - 0022-5304 (Linking)
VI  - 94
IP  - 3
DP  - 1991 Jun
TI  - Study on the pathogenicity of Campylobacter jejuni by modifying the medium.
PG  - 175-9
AB  - Campylobacter jejuni has been documented as one of the major aetiological agents 
      of diarrhoeal illness all over the world. Studies revealed its pathogenicity by 
      different assay methods, but none could be strongly recommended as a tool for 
      differentiating toxigenic strains of C. jejuni. This study was an attempt to 
      demonstrate better its pathogenicity by media modification. Fifteen isolates of 
      C. jejuni recovered from diarrhoeal patients at the International Centre for 
      Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Dhaka Hospital were included 
      in this study. The standard medium for C. jejuni was modified by the 
      incorporation of FeCl3 at different concentrations. The pathogenicity of the test 
      isolates were studied by rabbit ileal loop assay; Chinese hamster ovary (CHO), 
      human epithelial cervical carcinoma (HeLa); Y-1 adrenal cell lines and suckling 
      mouse assay. Sonicated extracts of the test organisms, grown with FeCl3 
      supplement, were also assayed. An enhanced growth of C. jejuni was obtained with 
      the increasing concentration of FeCl3 supplementation in the medium. Only five 
      isolates of C. jejuni produced cytotoxic effect on HeLa cell monolayer. Other 
      cell lines were not affected by the test specimens or sonicates. Rabbit ileal 
      loop assay did not reveal any fluid accumulation but on dissection, the test 
      loops were found highly haemorrhagic. No heat-stable (ST) toxin could be 
      detected. Cell-free culture supernatant of patients' isolates of C. jejuni had an 
      effect on HeLa cell monolayer. Sonicated extracts of patients' extracts had a 
      greater effect on HeLa cell monolayer. Pathogenic strains of C. jejuni might be 
      distinguished on HeLa cell monolayer using its sonicated extracts.
FAU - Siddique, A B
AU  - Siddique AB
AD  - Department of Microbiology, University of Dhaka, Bangladesh.
FAU - Akhtar, S Q
AU  - Akhtar SQ
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Trop Med Hyg
JT  - The Journal of tropical medicine and hygiene
JID - 0406044
RN  - 0 (Chlorides)
RN  - 0 (Culture Media)
RN  - 0 (Ferric Compounds)
RN  - U38V3ZVV3V (ferric chloride)
SB  - IM
MH  - Animals
MH  - Bacteriological Techniques
MH  - Campylobacter jejuni/growth & development/*pathogenicity
MH  - Chlorides
MH  - Culture Media/*chemistry
MH  - Diarrhea/*microbiology
MH  - Ferric Compounds
MH  - Rabbits
EDAT- 1991/06/01 00:00
MHDA- 1991/06/01 00:01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 1991/06/01 00:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
PST - ppublish
SO  - J Trop Med Hyg. 1991 Jun;94(3):175-9.