PMID- 20515652
OWN - NLM
STAT- MEDLINE
DCOM- 20100812
LR  - 20131121
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 397
IP  - 3
DP  - 2010 Jul 2
TI  - Berberine inhibits PTP1B activity and mimics insulin action.
PG  - 543-7
LID - 10.1016/j.bbrc.2010.05.153 [doi]
AB  - Type 2 diabetes patients show defects in insulin signal transduction that include 
      lack of insulin receptor, decrease in insulin stimulated receptor tyrosine kinase 
      activity and receptor-mediated phosphorylation of insulin receptor substrates 
      (IRSs). A small molecule that could target insulin signaling would be of 
      significant advantage in the treatment of diabetes. Berberine (BBR) has recently 
      been shown to lower blood glucose levels and to improve insulin resistance in 
      db/db mice partly through the activation of AMP-activated protein kinase (AMPK) 
      signaling and induction of phosphorylation of insulin receptor (IR). However, the 
      underlying mechanism remains largely unknown. Here we report that BBR mimics 
      insulin action by increasing glucose uptake ability by 3T3-L1 adipocytes and L6 
      myocytes in an insulin-independent manner, inhibiting phosphatase activity of 
      protein tyrosine phosphatase 1B (PTP1B), and increasing phosphorylation of IR, 
      IRS1 and Akt in 3T3-L1 adipocytes. In diabetic mice, BBR lowers hyperglycemia and 
      improves impaired glucose tolerance, but does not increase insulin release and 
      synthesis. The results suggest that BBR represents a different class of 
      anti-hyperglycemic agents.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Chen, Chunhua
AU  - Chen C
AD  - Drug Discovery Lab, School of Pharmacy, Shanghai University of Traditional 
      Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.
FAU - Zhang, Yuebo
AU  - Zhang Y
FAU - Huang, Cheng
AU  - Huang C
LA  - eng
PT  - Journal Article
DEP - 20100531
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 0I8Y3P32UF (Berberine)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 1)
RN  - EC 3.1.3.48 (Ptpn1 protein, mouse)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*drug effects/metabolism
MH  - Animals
MH  - Berberine/*administration & dosage
MH  - Diet/adverse effects
MH  - Female
MH  - Hyperglycemia/metabolism
MH  - Hypoglycemic Agents/*administration & dosage
MH  - Insulin/*metabolism/pharmacology
MH  - Insulin Resistance
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscle Cells/*drug effects/metabolism
MH  - Obesity/etiology/metabolism
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 1/*antagonists & inhibitors
EDAT- 2010/06/03 06:00
MHDA- 2010/08/13 06:00
CRDT- 2010/06/03 06:00
PHST- 2010/05/19 00:00 [received]
PHST- 2010/05/27 00:00 [accepted]
PHST- 2010/06/03 06:00 [entrez]
PHST- 2010/06/03 06:00 [pubmed]
PHST- 2010/08/13 06:00 [medline]
AID - S0006-291X(10)01083-1 [pii]
AID - 10.1016/j.bbrc.2010.05.153 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2010 Jul 2;397(3):543-7. doi: 
      10.1016/j.bbrc.2010.05.153. Epub 2010 May 31.