PMID- 20516188
OWN - NLM
STAT- MEDLINE
DCOM- 20100901
LR  - 20191210
IS  - 1559-6095 (Electronic)
IS  - 1559-6095 (Linking)
VI  - 2010
IP  - 6
DP  - 2010 Jun
TI  - Tracking and quantitative analysis of dynamic movements of cells and particles.
PG  - pdb.top80
LID - 10.1101/pdb.top80 [doi]
AB  - Understanding complex cellular processes requires investigating the underlying 
      mechanisms within a spatiotemporal context. Although cellular processes are 
      dynamic in nature, most studies in molecular cell biology are based on fixed 
      specimens, for example, using immunocytochemistry or fluorescence in situ 
      hybridization (FISH). However, breakthroughs in fluorescence microscopy imaging 
      techniques, in particular, the discovery of green fluorescent protein (GFP) and 
      its spectral variants, have facilitated the study of a wide range of dynamic 
      processes by allowing nondestructive labeling of target structures in living 
      cells. In addition, the tremendous improvements in spatial and temporal 
      resolution of light microscopes now allow cellular processes to be analyzed in 
      unprecedented detail. These state-of-the-art imaging technologies, however, 
      provide a huge amount of digital image data. To cope with the enormous amount of 
      image data and to extract reproducible as well as quantitative information, 
      computer-based image analysis is required. In this article, we describe methods 
      for computer-based analysis of multidimensional live cell microscopy images and 
      their application to study the dynamics of cells and particles. First, we sketch 
      a general workflow for quantitative analysis of live cell images. Then, we detail 
      computational methods for automatic image analysis comprising image 
      preprocessing, segmentation, registration, tracking, and classification. We 
      conclude with a discussion of quantitative analysis and systems biology.
FAU - Rohr, Karl
AU  - Rohr K
FAU - Godinez, William J
AU  - Godinez WJ
FAU - Harder, Nathalie
AU  - Harder N
FAU - Worz, Stefan
AU  - Worz S
FAU - Mattes, Julian
AU  - Mattes J
FAU - Tvarusko, Wolfgang
AU  - Tvarusko W
FAU - Eils, Roland
AU  - Eils R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cold Spring Harb Protoc
JT  - Cold Spring Harbor protocols
JID - 101524530
SB  - IM
MH  - Animals
MH  - *Cell Movement
MH  - Cell Nucleus/metabolism
MH  - Cells/*metabolism
MH  - Chlorocebus aethiops
MH  - HIV-1/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Image Processing, Computer-Assisted/*methods
MH  - Systems Biology
MH  - Vero Cells
EDAT- 2010/06/03 06:00
MHDA- 2010/09/02 06:00
CRDT- 2010/06/03 06:00
PHST- 2010/06/03 06:00 [entrez]
PHST- 2010/06/03 06:00 [pubmed]
PHST- 2010/09/02 06:00 [medline]
AID - 2010/6/pdb.top80 [pii]
AID - 10.1101/pdb.top80 [doi]
PST - ppublish
SO  - Cold Spring Harb Protoc. 2010 Jun;2010(6):pdb.top80. doi: 10.1101/pdb.top80.