PMID- 20518743
OWN - NLM
STAT- MEDLINE
DCOM- 20101116
LR  - 20120802
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 34
IP  - 9
DP  - 2010 Sep
TI  - Age-related association of rDNA and telomeres with the nuclear matrix in mouse 
      hepatocytes.
PG  - 925-31
LID - 10.1042/CBI20090457 [doi]
AB  - Transcribed sequences have been suggested to be associated with the nuclear 
      matrix, differing from non-transcribing sequences, which have been reported to be 
      contained in DNA loops. However, although a dozen of genes have their expression 
      level affected by aging, data on chromatin-nuclear matrix interactions under this 
      physiological condition are still scarce. In the present study, liver imprints 
      from young, adult and old mice were subjected to FISH (fluorescence in situ 
      hybridization) for 45S rDNA and telomeric sequences, with or without a lysis 
      treatment to produce extended chromatin fibres. There was an increased amount of 
      45S rDNA sequences located in DNA loops as the animals grow older, while 
      telomeric sequences were always observed in DNA loops irrespective of the animal 
      age. We assume that active rRNA genes associate with the nuclear matrix, while 
      DNA loops contain silent sequences. Transcription of each 45S rDNA repeat unit is 
      suggested to be dependent on its interaction with the nuclear matrix.
FAU - Moraes, Alberto S
AU  - Moraes AS
AD  - Department of Cell Biology, Institute of Biology, State University of Campinas, 
      13083-863 Campinas, Sao Paulo, Brazil. albertomoraes@icbim.ufu.br
FAU - Mondin, Mateus
AU  - Mondin M
FAU - Beletti, Marcelo E
AU  - Beletti ME
FAU - Aguiar-Perecin, Margarida L R
AU  - Aguiar-Perecin ML
FAU - Guaraldo, Ana Ma
AU  - Guaraldo AM
FAU - Mello, Maria Luiza S
AU  - Mello ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Chromatin)
RN  - 0 (DNA, Ribosomal)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Base Sequence
MH  - Cell Fractionation
MH  - Chromatin/metabolism
MH  - DNA/metabolism
MH  - DNA, Ribosomal/*metabolism
MH  - Hepatocytes/cytology/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Nuclear Matrix/*metabolism
MH  - Telomere/*metabolism
EDAT- 2010/06/04 06:00
MHDA- 2010/11/17 06:00
CRDT- 2010/06/04 06:00
PHST- 2010/06/04 06:00 [entrez]
PHST- 2010/06/04 06:00 [pubmed]
PHST- 2010/11/17 06:00 [medline]
AID - CBI20090457 [pii]
AID - 10.1042/CBI20090457 [doi]
PST - ppublish
SO  - Cell Biol Int. 2010 Sep;34(9):925-31. doi: 10.1042/CBI20090457.