PMID- 20519478
OWN - NLM
STAT- MEDLINE
DCOM- 20101104
LR  - 20211020
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 48
IP  - 8
DP  - 2010 Aug
TI  - Detection of human parechoviruses from clinical stool samples in Aichi, Japan.
PG  - 2683-8
LID - 10.1128/JCM.00086-10 [doi]
AB  - Between April 1999 and March 2008, a total of 4,976 stool specimens collected 
      from patients with suspected viral infection through infectious agent 
      surveillance in Aichi, Japan, were tested for the presence of human 
      parechoviruses (HPeVs). We detected HPeVs in 110 samples by either cell culture, 
      reverse transcriptase PCR (RT-PCR), or both. Serotyping either by neutralization 
      test or by nucleotide sequence determination and phylogenetic analysis of the VP1 
      region and 5' untranslated region (5'UTR) regions revealed that 63 were HPeV type 
      1 (HPeV-1), followed by 44 HPeV-3 strains, 2 HPeV-4 strains, and 1 HPeV-6 strain. 
      The high nucleotide and amino acid sequence identities of the Japanese HPeV-3 
      isolates in 2006 to the strains previously reported from Canada and Netherlands 
      confirmed the worldwide prevalence of HPeV-3 infection. Ninety-seven percent of 
      the HPeV-positive patients were younger than 3 years, and 86.2% younger than 12 
      months. The clinical diagnoses of HPeV-positive patients were gastroenteritis, 
      respiratory illness, febrile illness, exanthema, "hand, foot, and mouth disease," 
      aseptic meningitis, and herpangina. Among 49 HPeV-positive patients with 
      gastroenteritis, 35 were positive with HPeV-1 and 12 with HPeV-3, and out of 25 
      with respiratory illness, 11 were positive with HPeV-1 and 14 with HPeV-3. HPeV-3 
      seemed to be an important etiological agent of respiratory infection of children. 
      While HPeV-1 was detected predominantly during fall and winter, the majority of 
      the HPeV-3 cases were detected during summer and fall. A different pattern of 
      clinical manifestations as well as seasonality suggested that there are different 
      mechanisms of pathogenesis between HPeV-1 and HPeV-3 infections.
FAU - Ito, Miyabi
AU  - Ito M
AD  - Division of Virology, Department of Microbiology and Medical Zoology, Aichi 
      Prefectural Institute of Public Health, Nagare 7-6, Tuji-machi, Kita-ku, Nagoya, 
      Aichi 462-8576, Japan. miyabi_itou@prefaichi.lg.jp
FAU - Yamashita, Teruo
AU  - Yamashita T
FAU - Tsuzuki, Hideaki
AU  - Tsuzuki H
FAU - Kabashima, Yuka
AU  - Kabashima Y
FAU - Hasegawa, Akiko
AU  - Hasegawa A
FAU - Nagaya, Satoko
AU  - Nagaya S
FAU - Kawaguchi, Mariko
AU  - Kawaguchi M
FAU - Kobayashi, Shinichi
AU  - Kobayashi S
FAU - Fujiura, Akira
AU  - Fujiura A
FAU - Sakae, Kenji
AU  - Sakae K
FAU - Minagawa, Hiroko
AU  - Minagawa H
LA  - eng
SI  - GENBANK/AB300926
SI  - GENBANK/AB300927
SI  - GENBANK/AB300928
SI  - GENBANK/AB300929
SI  - GENBANK/AB300930
SI  - GENBANK/AB300931
SI  - GENBANK/AB300932
SI  - GENBANK/AB300933
SI  - GENBANK/AB300934
SI  - GENBANK/AB300935
SI  - GENBANK/AB300936
SI  - GENBANK/AB300937
SI  - GENBANK/AB300938
SI  - GENBANK/AB300939
SI  - GENBANK/AB300940
SI  - GENBANK/AB300941
SI  - GENBANK/AB300942
SI  - GENBANK/AB300943
SI  - GENBANK/AB300944
SI  - GENBANK/AB300945
SI  - GENBANK/AB300946
SI  - GENBANK/AB300947
SI  - GENBANK/AB300948
SI  - GENBANK/AB300949
SI  - GENBANK/AB300950
SI  - GENBANK/AB300951
SI  - GENBANK/AB300952
SI  - GENBANK/AB300953
SI  - GENBANK/AB300954
SI  - GENBANK/AB300955
SI  - GENBANK/AB300956
SI  - GENBANK/AB300957
SI  - GENBANK/AB300958
SI  - GENBANK/AB300959
SI  - GENBANK/AB300960
SI  - GENBANK/AB300961
SI  - GENBANK/AB300962
SI  - GENBANK/AB300963
SI  - GENBANK/AB300964
SI  - GENBANK/AB300965
SI  - GENBANK/AB300966
SI  - GENBANK/AB300967
SI  - GENBANK/AB300968
SI  - GENBANK/AB300969
SI  - GENBANK/AB300970
SI  - GENBANK/AB300971
SI  - GENBANK/AB300972
SI  - GENBANK/AB300973
SI  - GENBANK/AB300974
SI  - GENBANK/AB300975
SI  - GENBANK/AB300976
SI  - GENBANK/AB300977
SI  - GENBANK/AB300978
SI  - GENBANK/AB300979
SI  - GENBANK/AB300980
SI  - GENBANK/AB300981
SI  - GENBANK/AB300982
SI  - GENBANK/AB300983
SI  - GENBANK/AB300984
SI  - GENBANK/AB300985
SI  - GENBANK/AB300986
SI  - GENBANK/AB469777
SI  - GENBANK/AB469778
SI  - GENBANK/AB469779
SI  - GENBANK/AB469780
SI  - GENBANK/AB469781
SI  - GENBANK/AB469782
PT  - Journal Article
DEP - 20100602
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Capsid Proteins)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Age Factors
MH  - Capsid Proteins/genetics
MH  - Cell Culture Techniques
MH  - Child, Preschool
MH  - Cluster Analysis
MH  - Feces/*virology
MH  - Female
MH  - Gastroenteritis/virology
MH  - Genotype
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Japan/epidemiology
MH  - Male
MH  - Molecular Sequence Data
MH  - Neutralization Tests
MH  - Parechovirus/growth & development/*isolation & purification
MH  - Picornaviridae Infections/*diagnosis/*epidemiology/pathology/virology
MH  - Polymorphism, Genetic
MH  - RNA, Viral/genetics
MH  - Respiratory Tract Infections/virology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Sequence Homology
MH  - Serotyping
MH  - Virology/methods
PMC - PMC2916555
EDAT- 2010/06/04 06:00
MHDA- 2010/11/05 06:00
PMCR- 2011/02/01
CRDT- 2010/06/04 06:00
PHST- 2010/06/04 06:00 [entrez]
PHST- 2010/06/04 06:00 [pubmed]
PHST- 2010/11/05 06:00 [medline]
PHST- 2011/02/01 00:00 [pmc-release]
AID - JCM.00086-10 [pii]
AID - 0086-10 [pii]
AID - 10.1128/JCM.00086-10 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2010 Aug;48(8):2683-8. doi: 10.1128/JCM.00086-10. Epub 2010 Jun 
      2.