PMID- 20519584
OWN - NLM
STAT- MEDLINE
DCOM- 20100830
LR  - 20151119
IS  - 1477-9137 (Electronic)
IS  - 0021-9533 (Linking)
VI  - 123
IP  - Pt 12
DP  - 2010 Jun 15
TI  - Splicing-independent recruitment of U1 snRNP to a transcription unit in living 
      cells.
PG  - 2085-93
LID - 10.1242/jcs.061358 [doi]
AB  - Numerous non-coding RNAs are known to be involved in the regulation of gene 
      expression. In this work, we analyzed RNAs that co-immunoprecipitated with human 
      RNA polymerase II from mitotic cell extracts and identified U1 small nuclear RNA 
      (snRNA) as a major species. To investigate a possible splicing-independent 
      recruitment of U1 snRNA to transcription units, we established cell lines having 
      integrated a reporter gene containing a functional intron or a splicing-deficient 
      construction. Recruitment of U snRNAs and some splicing factors to transcription 
      sites was evaluated using fluorescence in situ hybridization (FISH) and 
      immunofluorescence. To analyze imaging data, we developed a quantitative 
      procedure, 'radial analysis', based on averaging data from multiple fluorescence 
      images. The major splicing snRNAs (U2, U4 and U6 snRNAs) as well as the U2AF65 
      and SC35 splicing factors were found to be recruited only to transcription units 
      containing a functional intron. By contrast, U1 snRNA, the U1-70K (also known as 
      snRNP70) U1-associated protein as well as the ASF/SF2 (also known as SFRS1) 
      serine/arginine-rich (SR) protein were efficiently recruited both to normally 
      spliced and splicing-deficient transcription units. The constitutive association 
      of U1 small nuclear ribonucleoprotein (snRNP) with the transcription machinery 
      might play a role in coupling transcription with pre-mRNA maturation.
FAU - Spiluttini, Beatrice
AU  - Spiluttini B
AD  - Ecole Normale Superieure, Institut de Biologie de l'ENS, IBENS, Paris, France.
FAU - Gu, Bo
AU  - Gu B
FAU - Belagal, Praveen
AU  - Belagal P
FAU - Smirnova, Anna S
AU  - Smirnova AS
FAU - Nguyen, Van Trung
AU  - Nguyen VT
FAU - Hebert, Charles
AU  - Hebert C
FAU - Schmidt, Ute
AU  - Schmidt U
FAU - Bertrand, Edouard
AU  - Bertrand E
FAU - Darzacq, Xavier
AU  - Darzacq X
FAU - Bensaude, Olivier
AU  - Bensaude O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Small Nuclear)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Ribonucleoprotein, U1 Small Nuclear)
RN  - 0 (SNRNP70 protein, human)
RN  - 0 (U1 small nuclear RNA)
RN  - 170974-22-8 (Serine-Arginine Splicing Factors)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Cell Line
MH  - Cells/cytology/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence, Multiphoton
MH  - Nuclear Proteins/genetics/metabolism
MH  - Protein Binding
MH  - RNA Polymerase II/genetics/*metabolism
MH  - *RNA Splicing
MH  - RNA, Small Nuclear/metabolism
MH  - RNA-Binding Proteins
MH  - Ribonucleoprotein, U1 Small Nuclear/genetics/*metabolism
MH  - Serine-Arginine Splicing Factors
MH  - *Transcription, Genetic
EDAT- 2010/06/04 06:00
MHDA- 2010/08/31 06:00
CRDT- 2010/06/04 06:00
PHST- 2010/06/04 06:00 [entrez]
PHST- 2010/06/04 06:00 [pubmed]
PHST- 2010/08/31 06:00 [medline]
AID - 123/12/2085 [pii]
AID - 10.1242/jcs.061358 [doi]
PST - ppublish
SO  - J Cell Sci. 2010 Jun 15;123(Pt 12):2085-93. doi: 10.1242/jcs.061358.