PMID- 20519866
OWN - NLM
STAT- MEDLINE
DCOM- 20100803
LR  - 20190724
IS  - 0031-6903 (Print)
IS  - 0031-6903 (Linking)
VI  - 130
IP  - 6
DP  - 2010 Jun
TI  - LC determination and pharmacokinetic study of hyperoside in rat plasma after 
      intravenous administration.
PG  - 873-9
AB  - A simple and specific high-performance liquid chromatographic (HPLC) method was 
      developed for the pharmacokinetic study of hyperoside (HP, isolated from the 
      leaves of Crataegus pinnatifida Bge. var. major) in rats after intravenous 
      administration. The plasma samples were deproteinized with methanol after 
      addition of internal standard (I.S.), baicalin. HPLC analysis was performed on a 
      Diamonsil C18 analytical column, using methanol-0.6% aqueous phosphoric acid 
      (45:55, v/v) as the mobile phase with UV detection at 340 nm. The calibration 
      curve was linear over the range of 0.8921-59.7125 microg/ml in rat plasma. The 
      average extraction recovery of HP was 99.33+/-0.86%, and the relative standard 
      deviations (R.S.D.s) of the intra- and inter-day precisions were no more than 7.8 
      and 2.5%, respectively. The lower limit of quantification (LLOQ) was 0.8921 
      microg/ml. The validated method was successfully applied during a pharmacokinetic 
      study in rats after intravenous administration of HP at different doses, and all 
      the results indicated that the pharmacokinetics of HP in rats obeyed nonlinear 
      processes.
FAU - Liu, Xun
AU  - Liu X
AD  - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, P. R. 
      China.
FAU - Wang, Dong
AU  - Wang D
FAU - Wang, Si-Yuan
AU  - Wang SY
FAU - Meng, Xian-Sheng
AU  - Meng XS
FAU - Zhang, Wen-Jie
AU  - Zhang WJ
FAU - Ying, Xi-Xiang
AU  - Ying XX
FAU - Kang, Ting-Guo
AU  - Kang TG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Yakugaku Zasshi
JT  - Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
JID - 0413613
RN  - 8O1CR18L82 (hyperoside)
RN  - 9IKM0I5T1E (Quercetin)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Crataegus/chemistry
MH  - Injections, Intravenous
MH  - Male
MH  - Plant Leaves/chemistry
MH  - Quercetin/*analogs & derivatives/blood/isolation & purification/pharmacokinetics
MH  - Rats
MH  - Rats, Wistar
EDAT- 2010/06/04 06:00
MHDA- 2010/08/04 06:00
CRDT- 2010/06/04 06:00
PHST- 2010/06/04 06:00 [entrez]
PHST- 2010/06/04 06:00 [pubmed]
PHST- 2010/08/04 06:00 [medline]
AID - JST.JSTAGE/yakushi/130.873 [pii]
AID - 10.1248/yakushi.130.873 [doi]
PST - ppublish
SO  - Yakugaku Zasshi. 2010 Jun;130(6):873-9. doi: 10.1248/yakushi.130.873.