PMID- 20521381
OWN - NLM
STAT- MEDLINE
DCOM- 20100812
LR  - 20211020
IS  - 1049-510X (Print)
IS  - 1049-510X (Linking)
VI  - 20
IP  - 1 Suppl 1
DP  - 2010 Winter
TI  - Glutamate transporters are differentially expressed in the hippocampus during the 
      early stages of one-day spatial learning task.
PG  - S1-28-32
AB  - INTRODUCTION: Uptake of glutamate in the hippocampus by specialized transporters 
      appears to be important for the prevention of glutamate-induced neurotoxicity. 
      However, the role of these transporters in synaptic plasticity and learning is 
      still unclear. We examined the expression pattern of glutamate transporters at 
      different stages of spatial learning using a one-day (three blocks) version of 
      the Morris Water Maze. METHODS: Male rats (Sprague Dawley, 3 months old) were 
      divided into three groups (learner, swim control, or naive control) and animals 
      were sacrificed after the first, second, or third block of training. The 
      hippocampi were immediately extracted and flash frozen for RNA analysis. Real 
      time polymerase chain reaction was employed to examine the expression of 
      glutamate transporter 1 (Glt-1), Glt1b, glutamate-aspartate transporter (GLAST) 
      and excitatory amino acid carrier-1 (EAAC1) in whole hippocampi. RESULTS: EAAC1 
      and GLAST RNA were downregulated in the learner and swimmer groups (compared to 
      naive) after the first two blocks of training during the one-day protocol but 
      EAAC1 returned to control levels by the end of the third block. GLAST levels were 
      upregulated by the third block of training. Glt-1b expression was downregulated 
      during the second block of training but returned to control by the third block. 
      CONCLUSIONS: The observed decreases in glutamate transporter expression may be 
      important during the early stages of spatial learning as a possible mechanism to 
      enhance glutamatergic availability during critical stages of learning. However, 
      similar decreases in glutamate transporter expression in both the learner and 
      swimmer groups indicate that the observed differences may be task-induced. 
      Additional experiments are currently underway to examine this possibility.
FAU - Fraticelli-Torres, Ada I
AU  - Fraticelli-Torres AI
AD  - Department of Physiology and Pharmacology; Ponce School of Medicine, 395 Zona 
      Industrial Reparada, Ponce, PR.
FAU - Matos-Ocasio, Felix
AU  - Matos-Ocasio F
FAU - Thompson, Kenira J
AU  - Thompson KJ
LA  - eng
GR  - R25 GM082406/GM/NIGMS NIH HHS/United States
GR  - ST32MH018882-21,22/ST/OHS HRSA HHS/United States
GR  - T32 MH018882/MH/NIMH NIH HHS/United States
GR  - 12RR003050/RR/NCRR NIH HHS/United States
GR  - S06 GM008239/GM/NIGMS NIH HHS/United States
GR  - GM082406/GM/NIGMS NIH HHS/United States
GR  - S06 GM008239-20S1/GM/NIGMS NIH HHS/United States
GR  - G12 MD007579/MD/NIMHD NIH HHS/United States
GR  - G12 RR003050/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ethn Dis
JT  - Ethnicity & disease
JID - 9109034
RN  - 0 (Amino Acid Transport System X-AG)
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 0 (Excitatory Amino Acid Transporter 3)
RN  - 0 (Slc1a1 protein, rat)
SB  - IM
MH  - Amino Acid Transport System X-AG/*metabolism
MH  - Animals
MH  - Down-Regulation/physiology
MH  - Excitatory Amino Acid Transporter 2/metabolism
MH  - Excitatory Amino Acid Transporter 3/metabolism
MH  - Hippocampus/*metabolism
MH  - Male
MH  - Maze Learning/*physiology
MH  - Polymerase Chain Reaction
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spatial Behavior/*physiology
PMC - PMC4064826
MID - NIHMS590427
EDAT- 2010/06/04 06:00
MHDA- 2010/08/13 06:00
PMCR- 2014/06/20
CRDT- 2010/06/04 06:00
PHST- 2010/06/04 06:00 [entrez]
PHST- 2010/06/04 06:00 [pubmed]
PHST- 2010/08/13 06:00 [medline]
PHST- 2014/06/20 00:00 [pmc-release]
PST - ppublish
SO  - Ethn Dis. 2010 Winter;20(1 Suppl 1):S1-28-32.