PMID- 20522122
OWN - NLM
STAT- MEDLINE
DCOM- 20101019
LR  - 20100720
IS  - 1365-2583 (Electronic)
IS  - 0962-1075 (Linking)
VI  - 19
IP  - 4
DP  - 2010 Aug
TI  - Apis mellifera has two isoforms of cytoplasmic HSP90.
PG  - 593-7
LID - 10.1111/j.1365-2583.2010.01015.x [doi]
AB  - Unlike most other insects, annotated genomic data suggest that Apis mellifera has 
      two homologous copies of the cytoplasmic gene HSP90. In this study, we did a 
      phylogenetic analysis on these two copies with some other insects HSP90 genes, 
      and we also manipulated a reverse transcript (RT)-PCR to find all the putative 
      transcripts for both copies. Phylogenetic analysis indicates that A. mellifera 
      possesses two isoforms of cytoplasmic HSP90: The 'traditional' isoform clusters 
      with cytoplasmic HSP90 of other insects. The other isoform, which occurs 
      phylogenetically as the sister group of all insects, may be a new gene and 
      specific to A. mellifera. The results of RT-PCR indicate that this new isoform 
      contains at least eight transcripts derived from the same genomic locus by 
      complicated alternative splicing (GenBank accession numbers: FJ713701, FJ713702, 
      FJ713703, FJ713704, FJ713705, FJ713706, FJ713707 and FJ713708, respectively). The 
      existence of this specific HSP90 might be related to the caste differentiation of 
      bees.
FAU - Xu, P J
AU  - Xu PJ
AD  - Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, 
      Chinese Academy of Sciences, Chaoyang District, Beijing, China.
FAU - Xiao, J H
AU  - Xiao JH
FAU - Xia, Q Y
AU  - Xia QY
FAU - Murphy, B
AU  - Murphy B
FAU - Huang, D W
AU  - Huang DW
LA  - eng
SI  - GENBANK/FJ713701
SI  - GENBANK/FJ713702
SI  - GENBANK/FJ713703
SI  - GENBANK/FJ713704
SI  - GENBANK/FJ713705
SI  - GENBANK/FJ713706
SI  - GENBANK/FJ713707
SI  - GENBANK/FJ713708
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100602
PL  - England
TA  - Insect Mol Biol
JT  - Insect molecular biology
JID - 9303579
RN  - 0 (HSP90 Heat-Shock Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Bees/*genetics
MH  - Cytoplasm/*genetics
MH  - Evolution, Molecular
MH  - Gene Expression Regulation
MH  - Genome/genetics
MH  - HSP90 Heat-Shock Proteins/*genetics/metabolism
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Protein Isoforms/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
EDAT- 2010/06/05 06:00
MHDA- 2010/10/20 06:00
CRDT- 2010/06/05 06:00
PHST- 2010/06/05 06:00 [entrez]
PHST- 2010/06/05 06:00 [pubmed]
PHST- 2010/10/20 06:00 [medline]
AID - IMB1015 [pii]
AID - 10.1111/j.1365-2583.2010.01015.x [doi]
PST - ppublish
SO  - Insect Mol Biol. 2010 Aug;19(4):593-7. doi: 10.1111/j.1365-2583.2010.01015.x. 
      Epub 2010 Jun 2.